If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf
MAK165
Fluorimetric Hydrogen Peroxide Assay Kit
sufficient for 500 fluorometric tests (red fluorescence)
Synonyma:
Hydrogen Peroxide Quantification Kit
Vybrat velikost
| Vám/Skladová položka | Dostupnost | Cena |
|---|---|---|
1 kit | Obraťte se na zákaznický servis a vyžádejte si informaci o dostupnosti. | 10 400,00 Kč |
O této položce
10 400,00 Kč
usage
sufficient for 500 fluorometric tests (red fluorescence)
detection method
fluorometric
relevant disease(s)
cardiovascular diseases; aging/geriatric diseases; orthopedic diseases; pulmonary disorders; neurological disorders
storage temp.
−20°C
General description
Application
Biochem/physiol Actions
Features and Benefits
1 of 1
Tato položka | |||
|---|---|---|---|
| usage sufficient for 500 fluorometric tests (red fluorescence) | usage - | usage sufficient for 500 fluorometric tests (infrared fluorescence) | usage sufficient for 200 fluorometric tests (green fluorescence) |
| detection method fluorometric | detection method colorimetric | detection method fluorometric | detection method fluorometric |
| storage temp. −20°C | storage temp. 2-8°C | storage temp. −20°C | storage temp. −20°C |
| relevant disease(s) cardiovascular diseases; aging/geriatric diseases; orthopedic diseases; pulmonary disorders; neurological disorders | relevant disease(s) cardiovascular diseases; aging/geriatric diseases; neonatal diseases; pediatric diseases; orthopedic diseases; neurological disorders; pulmonary disorders | relevant disease(s) orthopedic diseases; cardiovascular diseases; aging/geriatric diseases; neurological disorders; pulmonary disorders | relevant disease(s) aging/geriatric diseases; neurological disorders; pulmonary disorders; orthopedic diseases; cardiovascular diseases |
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signalword
Danger
hcodes
Hazard Classifications
Acute Tox. 4 Oral - Resp. Sens. 1
Skladovací třída
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
wgk
WGK 3
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How can I determine the shelf life / expiration / retest date of this product?
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How is shipping temperature determined? And how is it related to the product storage temperature?
1 answer-
Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
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Can we use Assay buffer MAK165C included in the kit to lyse cells to make cellular extract to measure endogenous H2O2 level.
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Yes, the Assay buffer that comes as one of the kit components (Product MAK165C) can be used to lyse cells. The kit provides 100 mL of Assay buffer, which is more than enough for the assays as well as to use some for sample preparation. MAK165C does not contain a surfactant, so the cells would need to be lysed by physical disruption...either sonication, or using a Dounce homogenizer (or equivalent).
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What is the limit of detection for H2O2?
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The lower limit of detection for kit MAK165 is 0.06 uM.
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Is the red peroxidase substrate in kit MAK165 the chemical 10-acetyl-3,7-dihydroxyphenoxazine (also known as Amplex Red)?
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The red peroxidase substrate in kit MAK165 is Amplex Red, which is the trademark of Invitrogen. The substrate is 10-Acetyl-3,7-dihydroxyphenoxazine and is chemically the same.
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Are black plates with black bottoms suitable for use with the MAK165 kit, even though the Technical Bulletin recommends black plates with clear bottoms?
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A solid black plate or a black wall with a clear bottom plate is suitable for use.
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Does MAK165 recognize all oxidized products or specifically hydrogen peroxide?
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MAK165 is used to quantify hydrogen peroxide in solutions and cell extracts. Therefore, the kit is specific for hydrogen peroxide and does not measure other oxidized products.
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