This kit is suitable for mammalian cell lysates, including those derived from mice. The sample can be frozen if needed, but aliquoting into smaller vials is recommended if multiple freeze/thaw cycles are anticipated.
MAK112
Arginase Activity Assay Kit
sufficient for 200 colorimetric tests
Synonyma:
Arginase Test Kit
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| Vám/Skladová položka | Dostupnost | Cena |
|---|---|---|
1 kit | Obraťte se na zákaznický servis a vyžádejte si informaci o dostupnosti. | 11 700,00 Kč |
O této položce
NACRES:
NA.84
UNSPSC Code:
12161503
11 700,00 Kč
Obraťte se na zákaznický servis a vyžádejte si informaci o dostupnosti.
Technický servis
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sufficient for 200 colorimetric tests
detection method
colorimetric
relevant disease(s)
cancer; immunological diseases; gastrointestinal diseases
storage temp.
−20°C
Gene Information
human ... ARG1(383), ARG2(384)
mouse ... ARG1(11846), ARG2(11847)
rat ... ARG2(29215), Arg1(29221)
General description
Arginase is a manganese-containing enzyme that catalyzes the conversion of arginine to urea and ornithine. Two isoforms of arginase are present in most mammals which differ in their tissue distribution and subcellular localization. Arginase I is a cytoplasmic protein predominantly expressed in the liver[1] where it catalyzes the fifth and final step of the Urea Cycle. Arginase II is a mitochondrial protein with broader tissue distribution whose function may include nitric oxide and polyamine metabolism. Decreased activity or expression of Arginase I results in the autosomal recessive disorder hyperarginemia.[1] Increased serum Arginase activity is implicated in hepatic injury and in certain pathological conditions such as cancer. Arginase also participates in multiple inflammatory reactions in the immune system, such as macrophage-mediated cytotoxicity.
Application
Suitable for the detection of arginase activity in enzyme preparations, serum, plasma, tissue culture, and similar samples. Suitable for studying the effects of drugs on arginase activity.
Arginase Activity Assay Kit has been used to assess the activity of arginase.
Biochem/physiol Actions
In this assay, arginase catalyzes the conversion of arginine to urea and ornithine. The urea produced specifically reacts with the substrate to generate a colored product, proportional to the arginase activity present.
Features and Benefits
Compatible with high-throughput handling systems.
1 of 1
Tato položka | |||
|---|---|---|---|
| Gene Information human ... ARG1(383), ARG2(384) | Gene Information human ... ARG1(383) | Gene Information human ... ARG2(384) | Gene Information human ... ARG2(384) |
| usage sufficient for 200 colorimetric tests | usage - | usage - | usage - |
| detection method colorimetric | detection method colorimetric | detection method - | detection method - |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| relevant disease(s) cancer; immunological diseases; gastrointestinal diseases | relevant disease(s) - | relevant disease(s) - | relevant disease(s) - |
signalword
Danger
hcodes
Hazard Classifications
Aquatic Chronic 3 - Eye Dam. 1 - Met. Corr. 1 - Repr. 1B - Skin Corr. 1A
Skladovací třída
6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects
wgk
WGK 2
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Is this kit suitable for cell lysate derived from mice and can the sample be frozen after lysing?
1 answer-
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How can I determine the shelf life / expiration / retest date of this product?
1 answer-
If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdfHelpful?
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How is shipping temperature determined? And how is it related to the product storage temperature?
1 answer-
Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
Helpful?
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Is an enzyme activation step of 55°C for 10 mins recommended if lysis was carried out at 4°C? If so, would the sample be mixed with the Mn solution first, followed by the arginine buffer after those 10 mins?
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The technical bulletin for this kit indicates to bring all reagents to room temperature prior to the assay and to preheat the arginine buffer to 37°C. The 5X substrate buffer is prepared by mixing arginine buffer with Mn solution in the described volumes shown in table 1. The assay reaction is performed by adding 10 uL of the previously prepared 5X substrate buffer to each of the sample wells. The incubation is performed at 2 hours at 37°C. Please review the technical bulletin for specific instructions on performing this assay.
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/314/808/mak112bul.pdf
There is no indication to heat the enzyme to 55°C or to mix your sample individually with Mn solution followed by arginine buffer.
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Is this kit suitable to work on nonhepatic (kidney, specifically) tissue derived from chickens?
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This product has not been specifically tested for use in chicken kidney. However, it has been used successfully in a number of tissue types, including lung, neural, adipose, and tumor. This kit has also been successfully used with HEK 293T cells, which originate from embryonic kidney. Suitability would have to be determined by the end user. Please see the link below to review the product datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/314/808/mak112bul.pdfHelpful?
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I would like to know whether this kit can be used with recombinant proteins and whether you recommend a specific one.
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This kit detects and measures the arginase activity, enzymatically. There are no anticipated problems detecting recombinant arginase, provided that the protein is enzymatically active.
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