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Key Documents

LY0100

Sigma-Aldrich

Lysozyme Activity Kit

sufficient for 100 tests

Synonyma:

Lysozyme assay kit

Přihlásitk zobrazení cen stanovených pro organizaci a smluvních cen


About This Item

UNSPSC Code:
12352202
NACRES:
NA.26

usage

sufficient for 100 tests

storage temp.

2-8°C

General description

The Lysozyme Detection Kit provides ready-to-use reagents for detecting the presence of lysozyme activity. This simple assay to detect lysozyme activity uses Micrococcus lysodeikticus cells as the substrate. Lysozyme activity results in the lysis of the Micrococcus lysodeikticus cells. During incubation of the lysozyme sample and substrate, the reaction is followed by monitoring the decrease in absorbance at 450 nm.

Application

Lysozyme Activity Kit has been used to measure the catalytic activity of native lysozyme, lysozyme purified from chicken egg white and lysozyme from pilocarpine-stimulated saliva.

Biochem/physiol Actions

Lysozyme hydrolytically cleaves the bonds between N-acetyl-D-glucosamine and N-acetylmuramic acid residues (GlcNAc β1-4Mur) in mucopolysaccharides and the glycan skeletons of the corresponding peptidoglycans (the latter are glycosaminoglycans linked to or crosslinked by peptide chains). Lysozyme effectively destroys the cell walls of bacteria.

Storage and Stability

The Reaction Buffer is stable for at least 2 years at 2–8 °C.
The Lysozyme (Catalog Number L6876) may be stored long term at –20 °C and remains active for 4 years.
The Micrococcus lysodeikticus cells (Catalog Number M3770) should be stored long term at –20 °C.

Součásti soupravy, které jsou dostupné také samostatně

Č. produktu
Popis
Bezpečností list

  • L6876Lysozyme from chicken egg white, powder or granules, ≥90 %, ≥39,000 units/mg proteinBezpečností list

  • Lysozyme Reaction Buffer

  • M3770Micrococcus lysodeikticus ATCC No. 4698, suitable for substrate for the assay of lysozyme, lyophilized cellsBezpečností list

pictograms

Health hazard

signalword

Danger

hcodes

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

flash_point_f

Not applicable

flash_point_c

Not applicable


Osvědčení o analýze (COA)

Vyhledejte osvědčení Osvědčení o analýze (COA) zadáním čísla šarže/dávky těchto produktů. Čísla šarže a dávky lze nalézt na štítku produktu za slovy „Lot“ nebo „Batch“.

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Navštívit knihovnu dokumentů

Tao Wang et al.
Chemical science, 7(5), 3234-3239 (2016-05-01)
Water-soluble allyl sulfones provide convenient site-specific disulfide rebridging of native proteins and cyclic peptides. The site-selective functionalization of (a) the peptide hormone somatostatin, (b) the interchain disulfide of bovine insulin and (c) functionalization of the proteins GFP and lysozyme with
Paula Chequer Gouveia Mól et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 1044-1045, 17-23 (2017-01-09)
In this study, a supermacroporous polyacrylamide cryogel was produced by cryo-polymerization and activated with Tris(hydroxymethyl)aminomethane (Tris-cryogel) to be applied as an affinity ligand for a one step purification of lysozyme (LYZ), directly from chicken egg white (EW). The Tris-cryogel presented
Silica Nanopollens Enhance Adhesion for Long-Term Bacterial Inhibition.
Song, H., et al.
Journal of the American Chemical Society, 138, 6455-6462 (2016)
Development of an affinity cryogel for one step purification of lysozyme from chicken egg white.
Mol P C G, et al.
Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, 1044, 17-23 (2017)
Modifications to the peptidoglycan backbone help bacteria to establish infection.
Davis K M and Jeffrey N W
Infection and Immunity, 799(2), 562-570 (2011)

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