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L9795

Lactate Oxidase from Aerococcus viridans

lyophilized powder

Synonyma:

L-lactate monooxygenase, lactate monooxygenase, lactate oxidative decarboxylase, lactate oxygenase, lactic oxidase, lactic oxygenase

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Vám/Skladová položkaDostupnostCena
50 units
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4 560,00 Kč
100 units
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11 600,00 Kč
500 units
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47 100,00 Kč

O této položce

NACRES:
NA.54
UNSPSC Code:
12352204
Biological source:
bacterial (Aerococcus viridans)

4 560,00 Kč


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Quality Segment

biological source

bacterial (Aerococcus viridans)

form

lyophilized powder

mol wt

80 kDa (gel filtration)

storage temp.

−20°C

General description

Lactate oxidase (LOX) is a flavin mononucleotide (FMN)-dependent enzyme and is a member of the α-hydroxy-acid oxidase flavoenzyme family.

Application

Lactate Oxidase from Aerococcus viridans has been used as a:
  • supplement in the culture medium to convert extracellular lactate to pyruvate
  • coating on glass beads for electrochemical detection of lactate from a single cell by a push-pull nozzle system
  • coating on multi-branched gold nanoparticles (GNPs) for the simultaneous colorimetric detection of lactate in saliva by paper-based multiplexed device

Biochem/physiol Actions

Lactate oxidase (LOX) catalyzes the oxidation of lactate to pyruvate and hydrogen peroxide in the presence of an FMN co-factor. The enzyme aids in the determination of L-lactate indirectly by measuring the hydrogen peroxide formed in the reaction.

Other Notes

One unit is defined as the amount of enzyme which generates 1m mole of Hydrogen Peroxide per minute at 37°C under standard assay conditions.

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Tato položka
G988859747G9637
biological source

bacterial (Aerococcus viridans)

biological source

-

biological source

-

biological source

bacterial (Pediococcus spp.)

form

lyophilized powder

form

lyophilized powder

form

powder

form

lyophilized powder

mol wt

80 kDa (gel filtration)

mol wt

-

mol wt

-

mol wt

~76 kDa (gel filtration)

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

2-8°C

Quality Level

200

Quality Level

200

Quality Level

100

Quality Level

200


pictograms

Health hazardExclamation mark

signalword

Danger

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Skladovací třída

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable



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Osvědčení o analýze (COA)

Lot/Batch Number

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Questions

1–2 of 2 Questions  
  1. What is the activity of this enzyme (U/g)?

    1 answer
    1. The minimum activity of this enzyme is 20 units per milligram. A survey of recent lots indicates a typical range of 45 - 50 units per milligram. This information is lot-specific and reported in the Certificate of Analysis. Please see the link below to review a sample or lot-specific Certificate:
      https://www.sigmaaldrich.com/product/sigma/l9795#product-documentation

      Helpful?

  2. Hello, I am a research chemist. I would kindly like to know the excipients present in Lactate Oxidase from Aerococcus viridans L9795. Thank you in advance.

    1 answer
    1. This freeze-dried preparation contains the following components:
      Recombinant Lactate Oxidase at 15-20%
      Sucrose at 50-84%
      Potassium Phosphate monobasic at 1-30%

      Helpful?

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