K4519
Keratinase
lyophilized powder
Synonyma:
Keratinase from Bacillus licheniformis, KerA, Keratinolytic protease
Přihlásitk zobrazení cen stanovených pro organizaci a smluvních cen
About This Item
Doporučené produkty
General description
Keratinase is a non-specific serine protease that cleaves non-terminal peptide bonds. This enzyme is active between 37°- 70°C. The highest activity is typically seen at 70°C.
Keratinase is also metalloprotease present in bacteria as well as eukaryotes. Keratinase isolated from Bacilus licheniformis culture is a monomeric enzyme.
Application
Keratinase may be used for enzymatic treatment of elementary body (EB), GAG molecules, and cells in the study of the role glycosaminoglycans (GAGs) in the invasion of host cells by Chlamydia pneumoniae strains.
Biochem/physiol Actions
Keratinase is a particular class of extracellular proteolytic inducible enzyme with the capability of degrading insoluble keratin substrates. It is important for hydrolyzing hair, feather, and collagen in sewage system during waste water treatment. It is also useful in food industry, animal feed preparation etc. Insoluble feather keratin from poultry industry may be converted by enzymatic hydrolysis to glues, feedstuffs, fertilizers, films or used for the production of rare amino acids serine, cysteine and proline.
Physical properties
Keratinase is activated by 0.10% SDS, 1.0% CTAB, and EDTA. Keratinase is partially inhibited by Tween®20, DMSO, isopropanol, methanol, and ethanol.
Unit Definition
One unit of enzyme is able to hydrolyze casein resulting in an absorbance value as the Folin-Ciocalteau reagent equivalent to 1 umole (181μg) of tyrosine per minute at pH 7.5 at 37 °C.
Preparation Note
The enzyme can be solubilized at 0.5-1.0 mg/mL in either sterile water or phosphate buffer. The best activity is seen with freshly prepared solutions. However, single-use aliquots of Keratinase solutions can be stored at -20° C.
Substrates
Keratin. Keratinases have also been used for the degradation of prion and prion-like proteins.
Other Notes
This enzyme contains a C-terminus 6-Histidine tag.
Legal Information
TWEEN is a registered trademark of Croda International PLC
signalword
Warning
hcodes
Hazard Classifications
Acute Tox. 4 Oral - Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
target_organs
Respiratory system
Storage Class
11 - Combustible Solids
wgk_germany
WGK 3
Osvědčení o analýze (COA)
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Zákazníci si také prohlíželi
X Lin et al.
Applied and environmental microbiology, 58(10), 3271-3275 (1992-10-01)
A keratinase was isolated from the culture medium of feather-degrading Bacillus licheniformis PWD-1 by use of an assay of the hydrolysis of azokeratin. Membrane ultrafiltration and carboxymethyl cellulose ion-exchange and Sephadex G-75 gel chromatographies were used to purify the enzyme.
Bacterial Keratinases: Useful Enzymes for Bioprocessing Agroindustrial Wastes and Beyond.
Brandelli A
Food and Bioprocess Technology, 1(2), 105-116 (2008)
Ellen J Beswick et al.
The Journal of infectious diseases, 187(8), 1291-1300 (2003-04-16)
The role of glycosaminoglycans (GAGs) in the invasion of host cells by Chlamydia pneumoniae strains TW-183 and A-03 was investigated and compared with the role of invasion by C. trachomatis serovars L2 and E. The quantities of epithelial and endothelial
Helena Gradisar et al.
Applied and environmental microbiology, 71(7), 3420-3426 (2005-07-08)
Based on previous screening for keratinolytic nonpathogenic fungi, Paecilomyces marquandii and Doratomyces microsporus were selected for production of potent keratinases. The enzymes were purified and their main biochemical characteristics were determined (molecular masses, optimal temperature and pH for keratinolytic activity
Keratinolytic activity from the broth of a feather-degrading thermophillic Streptomyces thermoviolaceus strain SD8.
Chitte RR, Nalawade VK, Dey S.
Letters in Applied Microbiology, 28, 131-136 (1999)
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