The accepted molar extinction coefficient for human IgG is ~210,000 cm-1M-1. The A0.1%280 value is 1.38.
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/100/203/i4506dat.pdf
I4506
IgG from human serum
reagent grade, ≥95% (SDS-PAGE), essentially salt-free, lyophilized powder
Synonym(s):
Human IgG
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| Size/SKU | Availability | Price |
|---|---|---|
10 mg | Available to ship TODAYfromMILWAUKEE | $301.00 |
50 mg | Available to ship TODAYfromMILWAUKEE | $301.00 |
100 mg | Available to ship TODAYfromMILWAUKEE | $523.00 |
500 mg | Available to ship TODAYfromMILWAUKEE | $2,000.00 |
About This Item
Conjugate:
unconjugated
Technique(s):
ELISA: suitable, dot immunobinding: suitable, immunoelectrophoresis: suitable, western blot: suitable
Application:
DIBA, ELISA, IEP, WB
Citations:
228
conjugate
unconjugated
Quality Segment
grade
reagent grade
assay
≥95% (SDS-PAGE)
form
essentially salt-free, lyophilized powder
composition
Protein, ≥90%
technique(s)
ELISA: suitable, dot immunobinding: suitable, immunoelectrophoresis: suitable, western blot: suitable
storage temp.
2-8°C
General description
Purified Human IgG is isolated from pooled normal human serum by fractionation and ion-exchange chromatography. The product is supplied as an essentially salt-free (less than 1% sodium), lyophilized powder.
Application
IgG from human serum was used as a standard at a concentration of 1.274 mg/ml in ELISA to determine antibody responses to Porphyromonas gingivalis and Prevotella intermedia.[1] It was used for immunolabeling of Vero, BHK and PtK2 cells at a concentration of 0.2 mg/ml.[2] IgG from human serum was used as a standard in SDS-PAGE of anti-Shigella LPS antibodies.[3]
Biochem/physiol Actions
IgG antibody subtype is the most abundant of serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections.[4]
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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This Item | |||
|---|---|---|---|
| conjugate unconjugated | conjugate unconjugated | conjugate unconjugated | conjugate unconjugated |
| form essentially salt-free, lyophilized powder | form essentially salt-free, lyophilized powder | form essentially salt-free, lyophilized powder | form essentially salt-free, lyophilized powder |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
| technique(s) ELISA: suitable, immunoelectrophoresis: suitable, dot immunobinding: suitable, western blot: suitable | technique(s) - | technique(s) ELISA: suitable | technique(s) - |
| composition Protein, ≥90% | composition - | composition - | composition - |
| grade reagent grade | grade reagent grade | grade reagent grade | grade reagent grade |
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Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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Hello, I was wondering if the molar extinction coefficient is available for the reference i4506-500mg? Thanks for your help
1 answer-
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１．本IgG試薬は、何人の健常人の血漿プールから得られたものでしょうか？ ２．本IgG試薬は医薬品「静注用免疫グロブリン（IVIG）」と同等品と考えてよろしいでしょうか？ 炎症抑制活性が確認された例はあるでしょうか？ 精製度はIVIGと比較してどうでしょうか？
1 answer-
The number of donors is not recorded for the original starting raw material. Historically, donor numbers for the plasma raw material are estimated to range from 1600 to 2000 individuals. No anti-inflammatory characterization has been performed. This product is intended for research use only. Unless otherwise stated this product is not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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What is the proportion of IgG1, IgG2, IgG3 and IgG4 in this product?
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In human serum, the 4 subclasses of human IgG are present at the approximate proportions of 66%, 23%, 7% and 4%, respectively.
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How is this standard reconstituted?
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The product is essentially a salt-free preparation and should be reconstituted to a minimum concentration of 1 mg/ml with a saline solution containing 150 mM NaCl.
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What is the expected MW of the product?
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The molecular weight of this product is not determined on a lot to lot basis. According to published literature, the molecular weight of Immunoglobulin G (IgG) is approximately 150 - 160 KDa. Historical data available for this product indicates that in SDS-PAGE electrophoresis this material will have multiple bands ranging from 155 kDa - 220 kDa.
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Necesito saber la secuencia de la proteína o el número para buscarla en uniprot
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The protein sequence is not determined for this product. Human IgG has 4 subclasses: IgG1, IgG2, IgG3 and IgG4. These are present in serum at approximately 66%, 23%, 7% and 4%, respectively.
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What is the isoelectric point of this
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This product is not tested on a lot-to-lot basis to determine the isoelectric point. However, historical data indicates an isoelectric point range of 6.1 - 8.5.
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Is product I4506, because it is derived from human serum, considered to potential biohazard?
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As indicated in the corresponding Data Sheet at the link below: "BIOHAZARD: Handle as if capable of transmitting infectious agents". The source material is tested and found negative for antibody to HIV, antibody to HCV, and for HbsAG.
Please see the link below to review the product data sheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/100/203/i4506dat.pdfHelpful?
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What is the recommended storage method for item I4506, IgG from human serum, after reconstitution?
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After reconstitution, keep aliquots at 2-8°C for short-term use (up to 4 weeks) and store the remaining aliquots at -20°C.
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What is the significance of absorbance at 280 nm (E280 1% = 14)?
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The absorbance at 280 nm (E280 1% = 14) indicates the percent extinction coefficient (ϵ1%).
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