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H8162

Millipore

HIS-Select® Cobalt Affinity Gel

(1:1 suspension in a 30% ethanol solution)

Synonyma:

cobalt affinity gel, cobalt charged agarose

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About This Item

UNSPSC Code:
41106500
NACRES:
NA.56

form

(1:1 suspension in a 30% ethanol solution)

shelf life

1 yr (when stored properly.)

technique(s)

protein purification: suitable

matrix

6% Beaded Agarose

capacity

>15 mg/mL, agarose binding capacity (protein)(with an approx. 30 kDa protein)

storage temp.

2-8°C

General description

The HIS-Select Cobalt Affinity Gel is a quadridentate chelate on beaded agarose that is charged with cobalt. The gel is designed to specifically bind histidine-containing proteins / His-tag proteins. It is selective for recombinant proteins with histidine tags and exhibits low non-specific binding of other proteins.
HIS-Select Cobalt Affinity Gel is an immobilized metal-ion affinity chromatography (IMAC) product. The matrix for this affinity gel is 6% beaded agarose. The selectivity can be modulated with the inclusion of imidazole during chromatography. HIS-Select Cobalt Affinity Gel is durable and can capture recombinant proteins with histidine tags at a high flow rate. Recombinant proteins with His tags are bound using native, denaturing, or mild reducing conditions. The capacity of this affinity gel is typically >15 mg/mL of packed gel, determined with a His-tagged protein of molecular mass ~30 kDa.

Application

HIS-Select Cobalt Affinity Gel allows for high purity, low non-specific binding, and high binding capacity of protein. HIS-Select Cobalt Affinity Gel also works well for purification of histidine-tagged proteins / His-tag proteins in native, denaturing, or mild reducing conditions. HIS-Select™ Cobalt Affinity Gel has been used to purify His-tagged proteins.

Physical form

1:1 suspension in a 30% ethanol solution

Legal Information

HIS-Select is a registered trademark of Merck KGaA, Darmstadt, Germany
HiScreen is a trademark of Cytiva

signalword

Danger

Hazard Classifications

Aquatic Acute 1 - Aquatic Chronic 1 - Carc. 1B - Eye Irrit. 2 - Flam. Liq. 3 - Muta. 2 - Repr. 1B - Resp. Sens. 1 - Skin Sens. 1

wgk_germany

WGK 3

ppe

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter

Osvědčení o analýze (COA)

Vyhledejte osvědčení Osvědčení o analýze (COA) zadáním čísla šarže/dávky těchto produktů. Čísla šarže a dávky lze nalézt na štítku produktu za slovy „Lot“ nebo „Batch“.

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