GE29-0915-98

Superose^® 6 Increase

3.2/300, Cytiva 29-0915-98, 8.6 μm avg. part. size

• Synonyma: Size exclusion chromatography, Superose 6 column
• NACRES: SB.52
• UNSPSC Code: 41115700

Vlastnosti

• material: glass column
• product line: Superose^®
• form: prepacked column
• crosslinking: cross-linked
• mol wt: protein M_r 40 MDa (exclusion limit), protein M_r 5-5000 kDa (fractionation range), M_p 1-300 kDa (dextrans fractionation range)
• packaging: pkg of 1 ea
• manufacturer/tradename: Cytiva 29-0915-98
• storage condition: (20% ethanol )
• parameter: 0.04 mL/min flow rate (recommended), 0.15 mL/min max. flow rate, 2 Mpa pressure drop required, 4-50 μL sample volume
• technique(s): liquid chromatography (LC): suitable
• bed diam. × H: 3.2 mm × 300 mm
• bed volume: 2.4 mL
• matrix: cross-linked agarose
• matrix active group: agarose
• avg. part. size: 8.6 μm
• cleaning: 1-14
• working range: 3-12
• separation technique: size exclusion (SEC)
• shipped in: ambient
• storage temp.: 2-30°C

Popis

General description

Superose^® 6 Increase 3.2/300 is a versatile, prepacked column for size exclusion chromatography in small-scale preparative purification (4 to 50 μl sample volume), as well as for characterization and analysis of proteins and other biomolecules with molecular weights between 5 000 and 5 000 000. When high resolution is required, sample amount is limited and low buffer consumption is important, this is the column of choice.

Application

Stable in all common buffers: 1 M acetic acid, 8 M urea, 6 M guanidine hydrochloride, 1% SDS, organic solvents, 0.5 M NaOH (for cleaning-in-place)

Features and Benefits

• Increased resolution compared with Superose 6, for higher purity and improved analysis results.
• Reduced runtime compared with Superose 6, for rapid results.
• Versatile use for both preparative and analytical purposes, especially for large proteins and complexes.
• Tolerance to repeated harsh cleaning procedures at high pH, giving long column lifetime and minimal carry-over.

Preparation Note

Storage Temp.: 4°C to 30°C

Store at 4 to 30 °C (20% Ethanol)

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Other Notes

Discover LiChropur reagents ideal for HPLC or LC-MS analysis

Legal Information

Superdex is a registered trademark of Cytiva

Superose is a registered trademark of Cytiva

Bezpečnostní informace

• Skladovací třída: 3 - Flammable liquids