GE17-5072-01
Q Sepharose™ Xl
Cytiva 17-5072-01, pack of 300 mL
About This Item
Doporučené produkty
ligand
quaternary amine
description
Ion Exchanger Type (value)
packaging
pack of 300 mL
manufacturer/tradename
Cytiva 17-5072-01
matrix
6% cross-linked agarose with dextran surface extenders
particle size
45-165 μm
average diameter
90 μm
cleaning in place
2-14
working range
2-12
suitability
suitable for bioprocess medium
Související kategorie
General description
Q Sepharose™ XL is based on a highly cross-linked, bead formed 6% agarose matrix similar to the well established Q Sepharose™ Fast Flow media. Dextran chains are covalently coupled to the agarose matrix and modified with quaternary ammonium (Q) strong anion exchange groups through chemically stable ether bonds. The stable agarose matrix and long, flexible chains of dextran with bound charged groups work together to increase loading capacity whilst allowing high flow rates.
With its high loading capacities and excellent physical and chemical stability, Q Sepharose™ XL is suitable for the capture stage of a downstream process, which is characterised by large loads of crude samples.
As member of the BioProcess media range, Q Sepharose™ XL meets industrial demands with security of supply and comprehensive technical and regulatory support.
Features and Benefits
- High loading capacities give more productive capture from clarified feedstocks.
- Strong anion exchanger designed for capture steps
- High chemical stability for effective CIP/sanitization
- The hydrophilic nature of the base matrix ensures low levels of non-specific binding leading to low levels of host cell-derived impurities in the elution pool.
Storage and Stability
Analysis Note
Legal Information
signalword
Warning
hcodes
Storage Class
3 - Flammable liquids
Osvědčení o analýze (COA)
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Sortimentní položky
This page shows Hydrophobic Interaction Chromatography (HIC) in a purification strategy.
This page shows volatile and non-volatile buffer suggestions for anion and cation exchange chromatography.
This page covers the standard ÄKTAdesign configurations for simple IEX chromatography.
This page covers practical problems that may lead to a non-ideal IEX separation and their solutions.
Protokoly
This page covers how and when to use Sepharose XL media for purification of proteins.
This page discusses how to do a purification with SOURCE™ media from Cytiva.
This page shows how to perform column packing and preparation for ion exchange chromatography and chromatafocusing when using Tricorn or XK columns available from Cytiva.
This page clarifies sample preparation, buffer exchange and desalting, removal of lipoproteins, phenol red, and low molecular weight contaminants in Ion exchange chromatography.
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