G0751

β-Glucuronidase from Helix pomatia

Type H-1, partially purified powder, ≥300,000 units/g solid

• Synonyma: β-D-Glucuronide glucuronosohydrolase
• Číslo CAS: 9001-45-0
• UNSPSC Code: 12352204
• NACRES: NA.54
• EC Number: 232-606-8
• MDL number: MFCD00130629
• Číslo EC: 3.2.1.31 (BRENDA, IUBMB)
• Specific activity: ≥300,000 units/g solid

Vlastnosti

• type: Type H-1
• Quality Segment: 200
• form: partially purified powder
• specific activity: ≥300,000 units/g solid
• secondary activity: ≥10,000 units/g solid sulfatase
• storage temp.: −20°C

Popis

General description

β-Glucuronidase Type H-1 from Helix pomatia is partially purified powder containing enzymes derived from the Roman snail. Many β-glucuronidases derived from mollusks also contain sulfatase activity.

Application

New Technical Article Comparing Performance of Different Enzymes
Learn more
about recent application data generated by Sigma R&D to optimize hydrolysis for different drug classes using enzymes from different sources and the use of a chromatographicaly purified enzyme to reduce the effect of esterase activity resulting in conversion of 6-MAM to Morphine

Biochem/physiol Actions

Used for the hydrolysis of glucuronide conjugates in urinary metabolite analysis

Analysis Note

Many β-glucuronidases derived from mollusks also contain sulfatase activity.

Other Notes

One Sigma or modified Fishman unit will liberate 1.0 μg of phenolphthalein from phenolphthalein glucuronide per hr at 37 °C at pH 5.0 (30 min assay).

Sulfatase Unit Definition: One unit of sulfatase will hydrolyze 1.0 μmole p-nitrocatechol sulfate per hr at pH 5.0 at 37 °C.

Bezpečnostní informace

• pictograms: GHS08
• signalword: Danger
• Skladovací třída: 11 - Combustible Solids
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)
• hcodes: H334
• pcodes: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• wgk: WGK 1