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D1878

2,6-Dichloroindophenol sodium salt hydrate

Powder, BioReagent

Synonyma:

2,6-Dichloro-N-(4-hydroxyphenyl)-1,4-benzoquinoneimine sodium salt, 2,6-Dichlorobenzenone-indophenol sodium salt, 2,6-Dichlorophenolindophenol sodium salt hydrate, DCIP, DPIP

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Vám/Skladová položkaDostupnostCena
5 g
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2 810,00 Kč
25 g
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11 700,00 Kč

O této položce

Empirický vzorec (Hillův zápis):
C12H6Cl2NNaO2 · xH2O
Číslo CAS:
Molekulová hmotnost:
290.08 (anhydrous basis)
NACRES:
NA.47
PubChem Substance ID:
UNSPSC Code:
12171500
EC Number:
210-640-4
MDL number:
Beilstein/REAXYS Number:
3641229

2 810,00 Kč


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Název produktu

2,6-Dichloroindophenol sodium salt hydrate, suitable for vitamin C determination, BioReagent

product line

BioReagent

Quality Segment

form

powder

solubility

H2O: soluble 10 mg/mL, clear, blue to very deep blue

suitability

suitable for vitamin C determination

application(s)

diagnostic assay manufacturing
food and beverages
hematology
histology

storage temp.

room temp

SMILES string

O.[Na+].[O-]c1ccc(cc1)\N=C2/C=C(Cl)C(=O)C(Cl)=C2

InChI

1S/C12H7Cl2NO2.Na.H2O/c13-10-5-8(6-11(14)12(10)17)15-7-1-3-9(16)4-2-7;;/h1-6,16H;;1H2/q;+1;/p-1

InChI key

XHSOLXWCGCVQHE-UHFFFAOYSA-M

General description

2,6-Dichloroindophenol sodium salt hydrate is a blue redox dye.

Application

2,6-Dichloroindophenol sodium salt hydrate is suitable for vitamin C determination. 2,6-Dichloroindophenol (DCIP) may be used in the following studies:
  • As redox dye to investigate the efficiency of gold nanoparticles (Au-NP) for enzymatic activity of glucose oxidase (GOx).
  • To compose UVB specific dosimeter.[1]
  • Quantification of vitamin C (L-ascorbic acid) in fruit juices.
  • As substrate in NQO1 assay to investigate the liver cytosolic NQO1 activity spectrophotometrically.[2]
  • Mitochondrial succinate CoQ oxidoreductase (complex II) activity assay.[3]

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Tato položka
119814B1125211761
suitability

suitable for vitamin C determination

suitability

-

suitability

-

suitability

-

solubility

H2O: soluble 10 mg/mL, clear, blue to very deep blue

solubility

-

solubility

H2O: 50 mg/mL

solubility

-

application(s)

diagnostic assay manufacturing
food and beverages
hematology
histology

application(s)

food and beverages

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

form

powder

form

solid

form

powder

form

powder

storage temp.

room temp

storage temp.

-

storage temp.

room temp

storage temp.

room temp

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200


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Skladovací třída

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)



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Osvědčení o analýze (COA)

Lot/Batch Number

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Questions

1–8 of 8 Questions  
  1. Other than use as a reagent for determination of Vitamin C, are there other reported uses of Product D1878, 2,6-Dichloroindophenol sodium salt hydrate?

    1 answer
    1. Yes. Conn's Biological Stains indicates Dichloroindophenol sodium salt has been used as an electron receptor, for use in demonstrating NADPH diaphorase and to stain glutathione reductase electrophoresis gels.

      Helpful?

  2. Are the absorbance maxima and the extinction coefficients available for Product D1878, 2,6-Dichloroindophenol sodium salt hydrate?

    1 answer
    1. Our Analytical Department has run absorbance spectra on many lots of D1878. Using 0.1 M phosphate buffer, pH 8.3 as the solvent, absorbance maxima in the range of 600-605 nm and a mM extinction coefficient of 18.0-19.7 have been reported.

      Helpful?

  3. Has Sigma Aldrich performed solution stability studies for Product D1878, 2,6-Dichloroindophenol sodium salt hydrate?

    1 answer
    1. No. Unfortunately, we have not performed solution stability studies on this product.

      Helpful?

  4. Are there published references for Dichloroindophenol sodium salt being used for the determination of Vitamin C?

    1 answer
    1. Yes. Please see: W. Horowitz ed. Official Methods of Analysis of the AOAC Washington D.C. , 777, (1970) and  K. Hiromi et al., Anal. Biochem. 101, 421, (1980).

      Helpful?

  5. Has Product D1878, 2,6-Dichloroindophenol sodium salt hydrate, been used as a pH or redox indicator?

    1 answer
    1. Yes. Conn's Biological Stains indicates Dichloroindophenol sodium salt in acid is  red and blue in alkali. The product is colorless when reduced.

      Helpful?

  6. Are there substances known to interfere in Vitamin C determination?

    1 answer
    1. Yes. Interfering substances are ferrous, stannous, and cuprous sulfite or thiosulfate ions, and SO2.

      Helpful?

  7. What is the solubility for Product D1878, 2,6-Dichloroindophenol sodium salt hydrate?

    1 answer
    1. During quality control testing, Sigma Aldrich tests solubility at 10 mg/mL in water.  Conn's Biological Stains, 10th Edition states that dichloroindophenol sodium salt is soluble up to 3% in water and up to 2% in ethanol.

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  8. What is the Department of Transportation shipping information for this product?

    1 answer
    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

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