PE/CA-PJ15

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 96121230, human tongue, squamous cell carcinoma. Epithelial-like morphology.

• Synonyma: Human PE/CA-PJ15, PE/CA-PJ15 Cell Line
• UNSPSC Code: 41106514
• Biological source: human mouth (epithelial cells)
• Relevant disease(s): cancer
• Growth mode: Adherent
• Karyotype: Not specified
• Morphology: Epithelial-like
• Manufacturer/tradename: ECACC

Vlastnosti

• biological source: human mouth (epithelial cells)
• manufacturer/tradename: ECACC
• packaging: tube of 5 μg 96121230-DNA-5UG, pkg of vial of cells 96121230-1VL
• growth mode: Adherent
• karyotype: Not specified
• morphology: Epithelial-like
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Popis

Application

Study of squamous cell carcinoma

Biochem/physiol Actions

Human oral squamous cell carcinoma

The cell line PE/CA-PJ15 was established from tongue tissue of a 45 year old male patient with oral squamous cell carcinoma. The cells have been shown to have epithelial differentiated characteristics expressing laminin and cytokeratins. Involucrin has been detected in some cells and cell groups.

Preparation Note

IDMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Split sub-confluent cultures (70-80%) 1:2 to 1:8 using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C. Note: cells may take up to 7 days to become confluent, a medium change during this period may be required.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.