LC-2/ad

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 94072247, human lung, Epithelial

• Synonyma: LC-2/ad Cells
• UNSPSC Code: 41106514
• Biological source: human lung
• Relevant disease(s): cancer
• Growth mode: Adherent
• Karyotype: Modal No 53-56
• Morphology: Epithelial
• Manufacturer/tradename: ECACC

Vlastnosti

• biological source: human lung
• manufacturer/tradename: ECACC
• packaging: tube of 5 μg 94072247-DNA-5UG, pkg of vial of cells 94072247-1VL
• growth mode: Adherent
• karyotype: Modal No 53-56
• morphology: Epithelial
• products: alpha-1-anti-trypsin
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Popis

Application

LC-2/ad has been used to study the ability of RXDX-105 as a therapy for cancers which are affected by RET (proto-oncogene tyrosine-protein kinase receptor) changes.

Biochem/physiol Actions

Established from the pleural effusion of a pulmonary adenocarcinoma in a 51 year-old Japanese female. LC-2/ad cells exhibit an epithelial appearance and a tendency to form small domes. Immunocytochemically the cells are positive for CEA and cytokeratins, including cytokeratin No.18. LC-2/ad produces at least two functionally active trypsin inhibitors together with several proteinases in vitro.

Human lung adenocarcinoma

Preparation Note

RPMI 1640:HAMS F12 (1:1) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS)

Split confluent cultures 1:2-1:4 ie seeding at 2-5 x 10,000 cells/cm² using 0.25% trypsin or trypsin/ EDTA ; 5%CO₂; 37°C

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.