SK-OV-3 Cell Line human

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 91091004, human ovary, Epithelial

• Synonyma: SKOV-3 Cells, SKOV3 Cells
• UNSPSC Code: 41106514
• Biological source: human ovary
• Relevant disease(s): cancer
• Growth mode: Adherent
• Karyotype: Hypodiploid to hypotetraploid
• Morphology: Epithelial
• Manufacturer/tradename: ECACC

Vlastnosti

• biological source: human ovary
• description: Human Caucasian ovary adenocarcinoma
• manufacturer/tradename: ECACC
• packaging: tube of 5 μg 91091004-DNA-5UG, pkg of vial of cells 91091004-1VL
• growth mode: Adherent
• karyotype: Hypodiploid to hypotetraploid
• morphology: Epithelial
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Popis

Application

SK-OV-3 cell line from humans has been used to study chimeric adenoviruses of Ad5 (species C) displaying proteins of the naturally enteric Ad40 (species F) as vectors for gene delivery.

Biochem/physiol Actions

Derived from the ascitic fluid from a 64 year old caucasian female with an ovarian tumour. Forms moderately well-differentiated adenocarcinoma consistent with ovarian primary cells.

Human Caucasian ovary adenocarcinoma

STR-PCR Data: Amelogenin: X
CSF1PO: 11
D13S317: 8,11
D16S539: 12
D5S818: 11
D7S820: 13,14
THO1: 9,9.3
TPOX: 8,11
vWA: 17,18

Preparation Note

McCoy′s 5a + 2mM Glutamine + 15% Foetal Bovine Serum (FBS).

Split sub-confluent cultures (70-80%) 1:2 to 1:3 i.e. seeding at 3-6x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.