SK-OV-3 Cell Line human

91091004, human ovary, Epithelial

• Synonyma: SKOV-3 Cells, SKOV3 Cells
• UNSPSC Code: 41106514

Vlastnosti

• product name: SK-OV-3 Cell Line human, 91091004
• biological source: human ovary
• description: Human Caucasian ovary adenocarcinoma
• growth mode: Adherent
• karyotype: Hypodiploid to hypotetraploid
• morphology: Epithelial
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Popis

Cell Line Origin

Human Caucasian ovary adenocarcinoma

Cell Line Description

Derived from the ascitic fluid from a 64 year old caucasian female with an ovarian tumour. Forms moderately well-differentiated adenocarcinoma consistent with ovarian primary cells.

Application

SK-OV-3 cell line from humans has been used to study chimeric adenoviruses of Ad5 (species C) displaying proteins of the naturally enteric Ad40 (species F) as vectors for gene delivery.

DNA Profile

STR-PCR Data: Amelogenin: X
CSF1PO: 11
D13S317: 8,11
D16S539: 12
D5S818: 11
D7S820: 13,14
THO1: 9,9.3
TPOX: 8,11
vWA: 17,18

Culture Medium

McCoy′s 5a + 2mM Glutamine + 15% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:2 to 1:3 i.e. seeding at 3-6x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Other Notes

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