C2C12 Cell Line from mouse

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 91031101, mouse muscle, Myoblast

• Synonyma: C2-C12 Cells
• UNSPSC Code: 41106514
• Biological source: mouse muscle
• Growth mode: Adherent
• Karyotype: Not specified
• Morphology: Myoblast
• Manufacturer/tradename: ECACC

Vlastnosti

• biological source: mouse muscle
• manufacturer/tradename: ECACC
• packaging: tube of 5 μg 91031101-DNA-5UG, pkg of vial of cells 91031101-1VL
• growth mode: Adherent
• karyotype: Not specified
• morphology: Myoblast
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• shipped in: dry ice
• storage temp.: −196°C

Popis

Application

C2C12 Cell Line has been used to study the the expression of OXT and OXTR (oxytocin and its receptor) genes and proteins and in differentiation studies.

Biochem/physiol Actions

Mouse C3H muscle myoblast

Subclone from myoblast line established from normal adult C3H mouse leg muscle. Differentiates rapidly; produces extensive contracting myotubes expressing characteristic muscle proteins. Provides model to study in vitro myogenesis and cell differentiation.

Preparation Note

DMEM + 2mM Glutamine + 10-15% Foetal Bovine Serum FBS / FCS.

Split semi-confluent cultures 1:3 to 1:6 i.e. seeding at 1-2x1,000 cells/cm² using 0.25% Trypsin or Trypsin/EDTA; 5% CO₂; 37°C. Do not allow cells to reach confluence.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.