TM4

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 88111401, mouse testis, Not specified

• UNSPSC Code: 41106514
• Biological source: mouse testis
• Growth mode: Adherent
• Karyotype: Not specified
• Morphology: Not specified

Vlastnosti

• biological source: mouse testis
• packaging: tube of 5 μg 88111401-DNA-5UG, pkg of vial of cells 88111401-1VL
• growth mode: Adherent
• karyotype: Not specified
• morphology: Not specified
• products: Transferrin, H-Y Ag, novel retinol-binding protein
• receptors: Androgen, oestrogen and progesterone receptors
• technique(s): cell culture | mammalian: suitable
• shipped in: dry ice
• storage temp.: −196°C

Popis

Application

Hormone studies

Biochem/physiol Actions

From primary culture of sertoli cell enriched preparations from normal testes of a 11-13 day old BALB/c nu/+ mice. Cells respond to FSH but not LH; Plasminogen activator secretion is stimulated by FSH and also retinoic acid. Note: only distributed frozen.

Mouse BALB/c testis sertoli cell

Preparation Note

Ham′s F12 + DMEM (1:1) + 2mM Glutamine + 5% Horse Serum (HS) + 2.5% Foetal Bovine Serum (FBS).

Split sub-confluent cultures (70-80%) 1:20 to 1:200 i.e. seeding at 1x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C. Cells resuscitated from frozen stock should be split 1:3 at the first passage and thereafter as above.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.