NCTC clone 929

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA. 88102702, mouse connective tissue, Fibroblast

• Synonyma: Earles′s Cells, L Cells, L-929 Cells, NCTC 929 Cells, NCTC-929L Cells
• UNSPSC Code: 41106514
• Biological source: mouse connective tissue
• Growth mode: Adherent
• Karyotype: 2n = 40
• Morphology: Fibroblast

Vlastnosti

• biological source: mouse connective tissue
• packaging: tube of 5 μg 88102702-DNA-5UG, vial of cells 88102702-1VL
• growth mode: Adherent
• karyotype: 2n = 40
• morphology: Fibroblast
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• shipped in: dry ice
• storage temp.: −196°C

Popis

Application

Replication, biochemical, neoplastic transformation, nutritional, metabolic enzyme, morphological, chromosome, immunologic, radiation, toxicologic and virus studies

Biochem/physiol Actions

From parental strain L which was derived from normal subcutaneous areolar and adipose tissue of 100 day old male C3H/An. Used in development of replicate culture techniques, chemically defined media, fluid suspension culture methods and virus assays. Susceptible to pseudorabies virus and vesicular stomatitis (Indiana strain) virus.

Mouse C3H/An connective tissue

Packaging

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

Preparation Note

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding at 1-3x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C. Freeze in 10% glycerol/90% FBS. After resuscitation cells may take up to 4 days to establish growth.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.