NIH-3T3 4-2

86041101, mouse skin, Fibroblast

• UNSPSC Code: 41106514

Vlastnosti

• Název produktu: NIH-3T3 4-2, NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA., 86041101
• biological source: mouse skin
• packaging: tube of 5 μg 86041101-DNA-5UG; pkg of vial of cells 86041101-1VL
• growth mode: Adherent
• karyotype: Not specified
• morphology: Fibroblast
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• shipped in: dry ice
• storage temp.: −196°C

Popis

General description

Product has lost contact inhibition

Cell Line Origin

Mouse fibroblast

Cell Line Description

A mouse fibroblast cell line which has an integrated mutant Moloney MuLV. The cell line can be used to generate helper-free defective retroviruses.

Application

Virus studies: Moloney MuLV, retroviruses

Culture Medium

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Other Notes

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