NIH-3T3 4-2

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

• UNSPSC Code: 41106514
• Biological source: mouse skin
• Growth mode: Adherent
• Karyotype: Not specified
• Morphology: Fibroblast

Vlastnosti

• biological source: mouse skin
• packaging: tube of 5 μg 86041101-DNA-5UG, pkg of vial of cells 86041101-1VL
• growth mode: Adherent
• karyotype: Not specified
• morphology: Fibroblast
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• shipped in: dry ice
• storage temp.: −196°C

Popis

General description

Product has lost contact inhibition

Application

Virus studies: Moloney MuLV, retroviruses

Biochem/physiol Actions

A mouse fibroblast cell line which has an integrated mutant Moloney MuLV. The cell line can be used to generate helper-free defective retroviruses.

Mouse fibroblast

Preparation Note

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.