Do-11-10

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 85082301, mouse unknown/unspecified, Not specified

• UNSPSC Code: 41106514
• Biological source: mouse unknown/unspecified
• Relevant disease(s): cancer
• Growth mode: Suspension
• Karyotype: Not specified
• Morphology: Not specified
• Manufacturer/tradename: ECACC

Vlastnosti

• biological source: mouse unknown/unspecified
• manufacturer/tradename: ECACC
• packaging: tube of 5 μg 85082301-DNA-5UG, pkg of vial of cells 85082301-1VL
• growth mode: Suspension
• karyotype: Not specified
• morphology: Not specified
• products: Not specified
• receptors: Not specified
• technique(s): cell culture: suitable using | mammalian
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Popis

Biochem/physiol Actions

Do-11-11 was produced by fusion of the antigen-specific I region-restricted (H-2d) BALB/c T-cell blasts with the AKR thyoma BW5147. This murine helper T cell hybridoma is specific for chicken ovalbumin (cOva) restricted to I-Ad and cross reacts with cOva I-antibody. The hybrid can be stimulated to secrete Interleukin 2 (IL-2) or to act as an antigen-specific helper T-cell. Expression of IL-2 can be lost by long-term culture of growth at high densities.

Mouse BALB/c (H-2d) T cells x Mouse AKR Thyoma, BW5147

Preparation Note

DMEM or RPMI 1640 + 2 mM Glutamine + 10% Fetal Bovine Serum (FBS).

Maintain cultures between 3-9 x 100,000 cells/ml; 5% CO₂; 37 °C. Cells should not be grown at high density; avoid long-term culture as this might lead to change of cell receptors.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.