Do-11-10

85082301, mouse unknown/unspecified, Not specified

• UNSPSC Code: 41106514

Vlastnosti

• product name: Do-11-10, from mouse unknown/unspecified, 85082301
• biological source: mouse unknown/unspecified
• growth mode: Suspension
• karyotype: Not specified
• morphology: Not specified
• products: Not specified
• receptors: Not specified
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Popis

Cell Line Origin

Mouse BALB/c (H-2d) T cells x Mouse AKR Thyoma, BW5147

Cell Line Description

Do-11-11 was produced by fusion of the antigen-specific I region-restricted (H-2d) BALB/c T-cell blasts with the AKR thyoma BW5147. This murine helper T cell hybridoma is specific for chicken ovalbumin (cOva) restricted to I-Ad and cross reacts with cOva I-antibody. The hybrid can be stimulated to secrete Interleukin 2 (IL-2) or to act as an antigen-specific helper T-cell. Expression of IL-2 can be lost by long-term culture of growth at high densities.

Culture Medium

DMEM or RPMI 1640 + 2 mM Glutamine + 10% Fetal Bovine Serum (FBS).

Subculture Routine

Maintain cultures between 3-9 x 100,000 cells/ml; 5% CO₂; 37 °C. Cells should not be grown at high density; avoid long-term culture as this might lead to change of cell receptors.

Other Notes

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