6906-50A

Human Peripheral Blood Monocytes: HPBM

• Synonym(s): HPBM, HPBM cells
• NACRES: NA.81
• UNSPSC Code: 41106514
• Biological source: human peripheral blood
• Growth mode: Suspension

Properties

• biological source: human peripheral blood
• form: liquid
• packaging: pkg of 5,000,000 cells
• manufacturer/tradename: Cell Applications, Inc
• growth mode: Suspension
• technique(s): cell culture | mammalian: suitable
• shipped in: dry ice
• storage temp.: −196°C

Description

General description

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Human Peripheral Blood Monocytes (HPBM) provide a useful in vitro tool for studying various aspects of pathology and biology. Our CD14⁺ Monocytes are isolated and purified from human peripheral blood of healthy donors and cryopreserved immediately after isolation.

HPBM are the precursors of tissue macrophages and dentritic cells and are central to immunological responses, including cell-mediated cytotoxicity and production of inflammatory mediators. The CD14 monocyte subset expresses a component of the lipopolysaccharide (LPS) receptor complex, are large with diameter of ~18 um, represent ~80%–90% of circulating monocytes, and express higher levels of CD62L (L-selectin) and CD11b (also referred to as Mac-1 or CR3) and lower levels of MHC class II than do CD16+ monocytes.

Application

Immune system dysfunction. Immunological responses, cell-mediated cytotoxicity, production of inflammatory mediators, hematopoiesis, myeloid differentiation, lipopolysaccharide (LPS), receptor complexes, CD62L (L-selectin), CD11b (Mac-1 or CR3), MHC class II.

Biochem/physiol Actions

Blood

Packaging

Immediately after isolation, 5,000,000 cells in DMEM containing 45% FBS & 10% DMSO.

Analysis Note

Each lot was tested for proper morphology, CD14 positive selection, Negative for HIV, Hepatitis B, Hepatitis C

Other Notes

DMEM containing 45% FBS & 10% DMSO

Disclaimer

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable