68268

Imidazole buffer Solution

BioUltra, 1 M in H₂O

• Synonyma: Imidazole solution, Glyoxaline solution
• Empirický vzorec (Hillův zápis): C₃H₄N₂
• Číslo CAS: 288-32-4
• Molekulová hmotnost: 68.08
• UNSPSC Code: 12161700
• NACRES: NA.25
• PubChem Substance ID: 57652144
• MDL number: MFCD00005183
• Beilstein/REAXYS Number: 103853
• eCl@ss: 39161001

Vlastnosti

• product line: BioUltra
• Quality Segment: 200
• form: liquid
• concentration: 1 M in H₂O
• impurities: DNases, none detected, RNases, none detected, insoluble matter, passes filter test, phosphatases, none detected, proteases, none detected
• pH: 9.5±0.5
• density: 1.01 g/mL at 20 °C
• SMILES string: c1c[nH]cn1
• InChI: 1S/C3H4N2/c1-2-5-3-4-1/h1-3H,(H,4,5)
• InChI key: RAXXELZNTBOGNW-UHFFFAOYSA-N

Popis

General description

Imidazole is used as a buffer in the pH range of 6.2-7.8 at 25°C. It is recommended as a component of a buffer for the assay of horseradish peroxidase and as a chelator for the binding of various divalent cations. Imidazole aids the elution of histidine-containing proteins from divalent cation resins (Sigma P6611, HisSelect.-HC Nickel affinity gel) and can also be used in reverse staining of SDS-PAGE gels for the detection of proteins.

Application

Imidazole buffer solution has been used as a component:

• of calmodulin-binding buffer 1 (CBB1) for the tandem affinity purification (TAP) of TAP-tagged proteins together with co-purifying proteins
• of lysis buffer, and SDS loading buffer for His-ubiquitin pull-down assays
• of imidazole wash and elution buffer for protein purification

Bezpečnostní informace

• pictograms: GHS08,GHS05
• signalword: Danger
• hcodes: H314,H360D
• pcodes: P201 - P280 - P301 + P330 + P331 - P303 + P361 + P353 - P305 + P351 + P338 + P310 - P308 + P313
• Hazard Classifications: Eye Dam. 1 - Repr. 1B - Skin Corr. 1C
• Skladovací třída: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• wgk: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter