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Key Documents

4381662

Sigma-Aldrich

iTRAQ® Reagent - 8PLEX One Assay Kit

Synonyma:

isobaric tags for relative and absolute quantitation 

Přihlásitk zobrazení cen stanovených pro organizaci a smluvních cen
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About This Item

UNSPSC Code:
12161503
NACRES:
NA.84

storage temp.

−20°C

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General description

Contains iTRAQ® Reagents 113, 114, 115, 116, 117, 118, 119 and 121, and also SDS Denaturant, TCEP Reducing Reagent, MMTS cysteine blocking reagent, TEAB buffer, and Isopropanol, for initial protocol development.

This kit contains the reagents in the equivalent SCIEX iChemistry® product number 4390811.
The iTRAQ® reagents are the first set of multiplexed, amine-specific, stable-isotope reagents that can label all peptides in up to eight different biological samples enabling simultaneous identification and quantitation, relative and absolute, while retaining important post-translational modifications (PTM) information.

Application

iTRAQ® Reagent - 8PLEX One Assay Kit has been used in the isobaric tag for relative and absolute quantitation (iTRAQ) labeling:
  • of digested total protein sample in mustard sprouts
  • of the total protein in broccoli sprouts
  • of the desalted peptides from different plants/intact chloroplasts

Features and Benefits

  • Simultaneously identify and quantify proteins from multiple samples
  • Expands protein and proteome coverage by labeling all peptides, including those with post-translational modifications (PTMs)
  • Increases confidence in identification and quantitation from MS/MS spectra by tagging multiple peptides per protein
  • Increases throughput and confidence in results for protein biomarker discovery studies
  • Offers a simple workflow without sample fractionation for reduced-complexity samples, such as affinity pull-downs
  • Provides the flexibility to multiplex up to eight different biological samples simultaneously in a single experiment
  • Fully supported by ProteinPilot Software on all SCIEX proteomics LC/MS/MS platforms

Analysis Note

To view the Protocol for the iTRAQ Reagents 8-plex Kit, please visit this Protocol link.

To view the Chemistry Quick Reference Card for the iTRAQ Reagents, please visit this Chemistry Quick Reference Card link.

Legal Information

iChemistry is a registered trademark of AB Sciex Pte. Ltd.
iTraq is a registered trademark of AB Sciex Pte. Ltd.

pictograms

FlameExclamation mark
GHS02,GHS07

signalword

Danger

Hazard Classifications

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Irrit. 2 - Flam. Liq. 2 - Skin Irrit. 2 - STOT SE 3

target_organs

Central nervous system

Storage Class

3 - Flammable liquids

wgk_germany

WGK 3

flash_point_c

2.0 °C - closed cup

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Xiaoming Ma et al.
Frontiers in veterinary science, 8, 663031-663031 (2021-09-18)
The plateau adaptability and stress resistance of yaks are widely known based on their capacity to survive under severe habitat conditions. However, a few studies on brain mitochondria have characterized these adaptations at the protein level. We identified and quantified
Yongqi Yin et al.
RSC advances, 11(20), 12336-12347 (2022-04-16)
Exogenous melatonin (10 μM) enhances ZnSO4 (4 mM) stress tolerance and regulates the isothiocyanate content of broccoli sprouts. Nevertheless, the molecular mechanism underlying the role of melatonin in isothiocyanate metabolism under ZnSO4 stress is unclear. The effects of exogenous melatonin
Peitong Wang et al.
Plant physiology, 178(4), 1568-1583 (2018-10-13)
Arsenic (As) is highly toxic to plants and detoxified primarily through complexation with phytochelatins (PCs) and other thiol compounds. To understand the mechanisms of As toxicity and detoxification beyond PCs, we isolated an arsenate-sensitive mutant of Arabidopsis (Arabidopsis thaliana), arsenate
Chao Cheng et al.
RSC advances, 10(10), 6052-6062 (2020-02-06)
Heat stress has been proved to increase the content of melatonin in plants. In the present study, a combination of methods including physiological and biochemical, gene transcription and proteomic were used to investigate the melatonin accumulation mechanisms in mustard sprouts
Amber E Alsop et al.
Nature communications, 6, 6841-6841 (2015-04-17)
During apoptosis, Bak permeabilizes mitochondria after undergoing major conformational changes, including poorly defined N-terminal changes. Here, we characterize those changes using 11 antibodies that were epitope mapped using peptide arrays and mutagenesis. After Bak activation by Bid, epitopes throughout the
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