17975

Ninhydrin reagent according to Stahl

for TLC derivatization

• NACRES: NA.22
• UNSPSC Code: 41116105

Vlastnosti

• Quality Segment: 100
• form: liquid
• technique(s): thin layer chromatography (TLC): suitable
• impurities: 1-butanol, acetic acid
• density: 0.823 g/mL at 20 °C

Popis

General description

Ninhydrin reagent is mostly used for the detection of amino acids. It basically gives a characteristic purple color on reaction with a-amino acids and other amino compounds, provided the pH of the reaction is 5.0 or higher. At an acid pH of 0.3, the reaction of ninhydrin with ornithinine and proline results in a red color product. Ninhydrin reagent can stay stable at room temperature in the absence of nitrogen atmosphere for upto 1 month in the ethylene glycol-sodium acetate solvent system.

Application

1 g ninhydrin dissolved in 475 mL 1-butanol and 25 mL acetic acid

Ninhydrin reagent may be used for the quantitative determination of total protein content in tissue hydrolysates.

Bezpečnostní informace

• pictograms: GHS02,GHS05,GHS07
• signalword: Danger
• hcodes: H226,H302,H315,H318,H335,H336
• pcodes: P210 - P233 - P280 - P301 + P312 - P303 + P361 + P353 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 4 Oral - Eye Dam. 1 - Flam. Liq. 3 - Skin Irrit. 2 - STOT SE 3
• target_organs: Central nervous system, Respiratory system
• Skladovací třída: 3 - Flammable liquids
• wgk: WGK 2
• flash_point_f: 93.2 °F - closed cup
• flash_point_c: 34 °C - closed cup
• ppe: Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter