MAB3864

Anti-DNA/Histone H1 Antibody

Chemicon^®, from mouse

• Synonyma: Anti-H1.4, Anti-H1E, Anti-H1F4, Anti-H1s-4, Anti-HIST1H1E, Anti-RMNS, Anti-dJ221C16.5
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: monoclonal
• Application: ELISA, FACS, ICC, IHC
• Citations: 39

Vlastnosti

• biological source: mouse
• Quality Segment: 100
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: monoclonal
• species reactivity (predicted by homology): all
• manufacturer/tradename: Chemicon^®
• technique(s): ELISA: suitable, flow cytometry: suitable, immunocytochemistry: suitable, immunohistochemistry: suitable
• isotype: IgG2a
• NCBI accession no.: NM_005321.2
• UniProt accession no.: P10412
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... H1-1(3024)

Popis

Immunogen

Human Raji cell nuclei

Application

Anti-DNA/Histone H1 Antibody is a high quality Mouse Monoclonal Antibody for the detection of DNA/Histone H1 & has been validated in ELISA, FC, ICC & IHC.

Immunocytochemistry: 5-10 μg/mL on 2% paraformaldehyde fixed cells (10 minutes at room temperature)

Immunohistochemistry: 5-10 μg/mL on paraformaldehyde or B5 fixed tissue sections.

Flow cytometry: 5-10 μg/mL using 2% paraformaldehyde fixed cells.

ELISA, direct and indirect

Optimal working dilutions must be determined by end user.

STAINING PATTERN:

Nuclear staining observed. Dense staining of nuclei. No staining of nucleoli.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Histones

Biochem/physiol Actions

Reacts with DNA/Histone 1. The antibody can be used to stain nuclei of all species and to identify necrosis. By immunohistochemistry, the antibody shows dense staining of nuclei with no staining of nucleoli observed.

Physical form

Format: Purified

Protein A purified culture supernatant. Liquid in PBS

Preparation Note

Maintain at -20°C in undiluted aliquots for up to 6 months from date of receipt. Avoid repeated freeze/thaw cycles.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Bezpečnostní informace

• Skladovací třída: 12 - Non Combustible Liquids
• wgk: WGK 2