MAB3300

Anti-TIMP-1 Antibody, clone 7-6C1

clone 7-6C1, Chemicon^®, from mouse

• Synonyma: Tissue Inhibitor of Metalloproteinase-1
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Vlastnosti

• biological source: mouse
• Quality Level: 100
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 7-6C1, monoclonal
• species reactivity: rat, human, bovine
• should not react with: mouse
• manufacturer/tradename: Chemicon^®
• technique(s): ELISA: suitable; immunohistochemistry: suitable; western blot: suitable
• isotype: IgG1κ
• NCBI accession no.: NM_003254.2
• UniProt accession no.: P01033
• shipped in: dry ice
• target post-translational modification: unmodified
• Gene Information: human ... TIMP1(7076)

Popis

General description

Tissue Inhibitors of Metalloproteinases (TIMPs) inhibit the proteolytic invasiveness of tumor cells and normal placental trophoblast cells. TIMP-1 and TIMP-2 have similar properties, specifically in inhibiting enzymes of matrix metalloproteinase family, and are thought to be of great importance in the maintenance of connective tissue integrity. TIMP-1 forms a complex of 1:1 stoichiometry with activated interstitial collagenase, activated stromelysin, active form of 72 kDa type IV collagenase (also known as MMP-2 or gelatinase A), and latent and active forms 92 kDa Type IV collagenase (also known as MMP-9 or gelatinase B). TIMP-1 levels in quiescent cells and tissues is low (pg/ml), and stimulation with the phorbol ester TPA or protein concentration is often needed to visualize protein bands. In addition, cell types differ greatly in the amount of TIMP-1 produced.

Specificity

MAB3300 is a purified mouse monoclonal antibody to bovine tissue inhibitor of metalloproteinases-1 (bovine TIMP-1). The antibody specifically reacts with bovine TIMP-1 and cross-reacts with human and rat TIMP-1, but not with mouse TIMP-1. By western blot the antibody recognizes a glycoprotein of 28.5 kDa, which is identified as tissue inhibitor of metalloproteinases-1 (TIMP-1).

Immunogen

Bovine TIMP-1

Application

Detect TIMP-1 using this Anti-TIMP-1 Antibody, clone 7-6C1 validated for use in ELISA, WB, IH.

Immunoblotting: 1:1000, identifies a band of approximately 30KDa. Note TIMP-1 is found in very low concentrations in most cells and tissues.Positive control is HT1080 cells treated with PMA or recombinant TIMP-1 protein CC3328.

Immunohistochemistry on frozen sections or acetone fixed tissues. Formalin fixed paraffin-embedded tissue sections are usually not reactive. PLP fixation is suggested as an alternative.

EIA

Optimal working dilutions must be determined by the end user.

Research Category
Cell Structure

Research Sub Category
MMPs & TIMPs

Linkage

Replaces: 04-1133

Physical form

Format: Purified

Purified immunoglobulin presented as a liquid in 0.1 M Na-Phosphate buffer, pH 7.0 containing protease free bovine serum albumin.

Storage and Stability

Maintain frozen at -20°C in undiluted aliquots for up to 12 months from date of receipt. Avoid repeated freeze-thaw cycles.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Bezpečnostní informace

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable