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Key Documents

MAB2624

Sigma-Aldrich

Anti-Endomucin Antibody, clone V.5C7

clone V.5C7, from rat

Synonyma:

Endomucin-2, Gastric cancer antigen Ga34, Mucin-14, MUC-14, Endomucin-1/2

Přihlásitk zobrazení cen stanovených pro organizaci a smluvních cen


About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rat

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

V.5C7, monoclonal

species reactivity

mouse

technique(s)

flow cytometry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG1κ

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

mouse ... Emcn(59308)

General description

Endomucin is an endothelial sialomucin belonging to a class of glycoproteins characterized by their high levels of O-glycosolation and rich quantities of sialic acid. It inhibits focal adhesion assembly and hinders extracellular matrix and cell interaction. Observed expression is highest in kidney heart, brain, liver, lung, spleen, and thymus. Endomucin is only found in endothelial cells.

Immunogen

Mouse endothelial cells

Application

Anti-Endomucin Antibody, clone V.5C7 detects level of Endomucin & has been published & validated for use in WB, IP, IH, FC.
Immunoprecipitation Analysis: A previous lot was used by an independent laboratory in IP of bEND cell lysate (Morgan, 1999).

Immunohistochemistry Analysis: A previous lot was used by an independent laboratory in immunhistochemical staining of frozen and paraffin-embedded tissue sections of mouse tongue, heart, uterus, brain, and small intestine (Morgan, 1999).

Flow Cytometry Analysis: A previous lot was used by an independent laboratory in FC of transfected COS-7 cells (Morgan, 1999).
Research Sub Category
Organelle & Cell Markers

Adhesion (CAMs)

Quality

Evaluated by Western Blot on mouse endothelioma cell line, bEND3, cell lysate.

Western Blot Analysis: 0.0025 µg/mL of this antibody detected Endomucin in 10 µg of bEND3 cell lysate.

Target description

~ 75kDa observed

Physical form

Format: Purified

Analysis Note

Control
bEND3 cell lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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