AB5084P
Anti-Dopamine D2 Receptor Antibody
Chemicon®, from rabbit
Synonyma:
Dopamine D2 receptor
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O této položce
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
polyclonal
Application:
ELISA, IHC, IP, WB
Citations:
152
Technický servis
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rabbit
Quality Segment
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
mouse, monkey, rat, human
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable, immunohistochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Gene Information
human ... DRD2(1813)
mouse ... Drd2(13489)
rat ... Drd2(24318)
rhesus monkey ... Drd2(694181)
General description
50 kDa
The Dopamine D2 Receptor is one of the five types (D1 to D5) of receptors for dopamine. The activity of this receptor is mediated by G proteins which inhibit adenylyl cyclase. 2 isoforms are reported for the D2 Dopamine Receptor, Isoform 1 and isoform 2 which are expressed in the pituitary and brain; Isoform 1 is expressed seven times more than isoform 2 in the caudate nucleus.
Immunogen
A 28 amino acid peptide sequence from the human D2 receptor within the cytoplasmic
loop #3.
loop #3.
Application
Research Category
Neuroscience
Neuroscience
Research Sub Category
Neurotransmitters & Receptors
Neurotransmitters & Receptors
This Anti-Dopamine D2 Receptor Antibody is validated for use in ELISA, IH, IP, WB for the detection of Dopamine D2 Receptor.
Western blot:
1-10 µg/mL (Chemiluminescence technique) The predicted size of D2 is approximately 50 kDa. However, additional bands at approximately 68 and 100 kDa, and others are typically seen in various tissues (Boundy et al. 1993; Sakata et al. 1992; Farooqui et al. 199; Farooqui & Prasad 1992, BBRC; Brock et al. 1992; Farooqui & Prasad 1992, Life Sci). Approximately 100-400 µg of membrane protein/lane should be loaded to visualize various bands.
Immunohistochemistry:
2-10 μg/mL on paraformaldehyde fixed sections on a previous lot.
Immunohistochemistry:
1-20 µg/mL on paraformaldehyde fixed sections.
Immunoprecipitation:
1-10 μg/mL using 100-400 μg of protein on a previous lot. An antibody made to this epitope has been shown to immunoprecipitate both the long and short forms (2).
ELISA:
0.5-1.0 μg/mL (1 μg/mL immunogen peptide (Catalog number AG221)/well) of a previous lot was used on ELISA.
Optimal working dilutions must be determined by the end user.
1-10 µg/mL (Chemiluminescence technique) The predicted size of D2 is approximately 50 kDa. However, additional bands at approximately 68 and 100 kDa, and others are typically seen in various tissues (Boundy et al. 1993; Sakata et al. 1992; Farooqui et al. 199; Farooqui & Prasad 1992, BBRC; Brock et al. 1992; Farooqui & Prasad 1992, Life Sci). Approximately 100-400 µg of membrane protein/lane should be loaded to visualize various bands.
Immunohistochemistry:
2-10 μg/mL on paraformaldehyde fixed sections on a previous lot.
Immunohistochemistry:
1-20 µg/mL on paraformaldehyde fixed sections.
Immunoprecipitation:
1-10 μg/mL using 100-400 μg of protein on a previous lot. An antibody made to this epitope has been shown to immunoprecipitate both the long and short forms (2).
ELISA:
0.5-1.0 μg/mL (1 μg/mL immunogen peptide (Catalog number AG221)/well) of a previous lot was used on ELISA.
Optimal working dilutions must be determined by the end user.
Biochem/physiol Actions
Specific for Dopamine Receptor D2 short and long form. Dopamine D2 receptor is known to exist in the short (D2S) and the long (D2L) forms that are encoded by splice variants of a single gene and differ only by the presence of an additional 29 amino acid in the intracellular, cytoplasmic loop 3 (1). D2L receptor is a 446 aa, G-protein coupled and transmembrane receptor protein (2). The immunogen peptide shows no significant homology with other dopamine receptors (D1 and D3-D5).
Physical form
ImmunoAffinity Purified
Purified rabbit polyclonal liquid in PBS with 0.1% BSA.
Preparation Note
Stable for 1 year at -20ºC from date of receipt.
Analysis Note
Control
Monkey brain tissue, rat brain lysate
Monkey brain tissue, rat brain lysate
Routinely evaluated by Western Blot on human brain lysates.
Western Blot Analysis: 1:1000 dilution of this lot detected Dopamine Receptor D2 on 10 μg of human brain lysates.
Western Blot Analysis: 1:1000 dilution of this lot detected Dopamine Receptor D2 on 10 μg of human brain lysates.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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wgk
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