07-690
Anti-Histone H3 Antibody, CT, pan
serum, Upstate®
Synonyma:
H3, Histone H3, H3 histone family, member T, histone 3, H3, histone cluster 3, H3
pro zobrazení organizačních a smluvních cen.
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O této položce
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Clone:
polyclonal
Species reactivity:
human, mouse, yeast, rat, chicken, Saccharomyces cerevisiae
Application:
ChIP, WB
Citations:
274
Technický servis
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rabbit
Quality Segment
antibody form
serum
antibody product type
primary antibodies
clone
polyclonal
species reactivity
human, mouse, yeast, rat, chicken, Saccharomyces cerevisiae
manufacturer/tradename
Upstate®
technique(s)
ChIP: suitable, western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Gene Information
human ... H3C1(8350)
mouse ... H3C1(360198)
rat ... H3C1(679994)
General description
Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.
Approx. 17 kDa
Immunogen
KLH-conjugated, synthetic peptide corresponding to the C-terminus of human Histone H3.
Application
Chromatin Immunoprecipitation:
This antibody has been reported by an independent laboratory to immunoprecipitate chromatin from yeast.
This antibody has been reported by an independent laboratory to immunoprecipitate chromatin from yeast.
Use Anti-Histone H3 Antibody, CT, pan (rabbit polyclonal antibody) validated in ChIP, WB to detect Histone H3 also known as H3, Histone H3, H3 histone family member T, histone 3 H3, histone cluster 3 H3.
Biochem/physiol Actions
Broad species cross-reactivity expected due to sequence homology.
Recognizes C-Terminal region of Histone H3, Mr 17 kDa
Physical form
Rabbit antiserum diluted 1:2 in storage buffer (0.02 M Phosphate, 0.25 M NaCl, 0.1% sodium azide) before the addition of glycerol to 30%.
Analysis Note
Routinely evaluated by immunoblot on acid extracted proteins from untreated, sodium butyrate or colcemid treated HeLa cells.
Western Blot Analysis:
1:25,000-1:50,000 dilution of this lot detected Histone H3 in a modification independent manner in acid extracted proteins from untreated, sodium butyrate or colcemid treated HeLa cells.
Western Blot Analysis:
1:25,000-1:50,000 dilution of this lot detected Histone H3 in a modification independent manner in acid extracted proteins from untreated, sodium butyrate or colcemid treated HeLa cells.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
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Skladovací třída
10 - Combustible liquids
wgk
WGK 1
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