06-1310

Anti-JAK2 Antibody

from rabbit

• Synonym(s): Janus kinase 2, Janus kinase 2 (a protein tyrosine kinase), tyrosine-protein kinase JAK2
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: polyclonal
• Application: IP, WB
• Citations: 5

Properties

• biological source: rabbit
• Quality Segment: 100
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: polyclonal
• species reactivity: mouse, human, rat
• technique(s): immunoprecipitation (IP): suitable, western blot: suitable
• NCBI accession no.: NP_001041642
• UniProt accession no.: Q62120
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... JAK2(3717) ; mouse ... Jak2(16452) ; rat ... Jak2(24514)

Description

General description

The Janus family of tyrosine kinases (JAK) includes JAK1, JAK2, JAK3 and Tyk2. This family associates with a variety of cytokine receptors and is activated by ligand binding. Upon ligand binding to cytokine receptors, JAKs phosphorylate themselves and their associated receptors. This provides multiple binding sites for signaling proteins containing SH2 or other phospho-tyrosine-binding domains, including STATs, Shc, insulin receptor substrates and FAK.

~130 kDa

Immunogen

Epitope: Protein kinase 1 domain

KLH-conjugated linear peptide corresponding to the protein kinase 1 domain of mouse JAK2.

Application

Research Category
Inflammation & Immunology

Signaling

Research Sub Category
Immunological Signaling

Kinases & Phosphatases

This Anti-JAK2 Antibody is validated for use in WB, IP for the detection of JAK2.

Western Blot Analysis: 1 µg/mL from a previous lot detected JAK2 in 10 µg of L6 cell lysate.

Immunoprecipitation Analysis: 10 µg from a previous lot immunoprecipitated JAK2 from RAW264.7 cell lysate.

Biochem/physiol Actions

This antibody recognizes the protein kinase 1 domain of JAK2.

Physical form

Format: Purified

Protein A purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
RAW264.7 cell lysate

Evaluated by Western Blot in RAW264.7 cell lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected JAK2 in 10 µg of RAW264.7 cell lysate.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable