05-801
Anti-trimethyl-Histone H3 (Lys36) Antibody, clone MC86, rabbit monoclonal
clone MC86, Upstate®, from rabbit
Synonyma:
H3K36me3, Histone H3 (tri methyl K36)
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O této položce
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Clone:
MC86, monoclonal
Species reactivity:
chicken
Application:
ChIP, DB, WB, inhibition assay
Citations:
8
Technický servis
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rabbit
Quality Segment
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
MC86, monoclonal
species reactivity
chicken
manufacturer/tradename
Upstate®
technique(s)
ChIP: suitable, dot blot: suitable, inhibition assay: suitable (peptide), western blot: suitable
isotype
IgG
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
trimethylation (Lys36)
General description
~17 kDa
Immunogen
peptide containing the sequence GVme3KKP, in which me3K corresponds to trimethyl lysine 36 of human histone H3
Application
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Histones
Histones
Use Anti-trimethyl-Histone H3 (Lys36) Antibody, clone MC86 (rabbit monoclonal antibody) validated in DB, WB, PIA to detect trimethyl-Histone H3 (Lys36) also known as H3K36me3, Histone H3 (tri methyl K36).
Biochem/physiol Actions
Broad species cross-reactivity is expected.
Recognizes Histone H3 trimethylated on Lysine 36.
Physical form
Format: Purified
Protein A Purified immunoglobulin in a solution containing 0.07M Tris-glycine, 0.105M NaCl, pH 7.4, 0.035% sodium azide, 30% glycerol.
Protein A purified
Preparation Note
Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Analysis Note
Control
Acid extracted proteins from HeLa cells
Acid extracted proteins from HeLa cells
routinely evaluated by immunoblot on chicken Core Histones (Catalog #13-107); did not detect unmodified recombinant Histone H3 (Catalog #14-411)
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Skladovací třída
10 - Combustible liquids
wgk
WGK 1
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