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Merck

05-577

Anti-PP2A Antibody, C subunit, demethylated, clone 4b7

ascites fluid, clone 4b7, Upstate®

Synonyma:

Anti-NEDLBA, Anti-PP2Ac, Anti-PP2CA, Anti-PP2Calpha, Anti-RP-C

Přihlásit pro zobrazení organizačních a smluvních cen.

Vybrat velikost

200 μL

11 000,00 Kč

11 000,00 Kč


K dispozici k odeslání DNESpodrobné informace


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O této položce

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702

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Název produktu

Anti-PP2A Antibody, C subunit, demethylated, clone 4b7, ascites fluid, clone 4b7, Upstate®

biological source

mouse

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

4b7, monoclonal

species reactivity

vertebrates

manufacturer/tradename

Upstate®

technique(s)

immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Quality Level

Gene Information

human ... PPP2CA(5515)

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Tato položka
05-54507-32407-250
species reactivity

vertebrates

species reactivity

mouse, Saccharomyces cerevisiae, human

species reactivity

bovine, rat, human, mouse

species reactivity

human, mouse, Xenopus, pig, rat

clone

4b7, monoclonal

clone

7A6, monoclonal

clone

polyclonal

clone

polyclonal

antibody form

ascites fluid

antibody form

culture supernatant

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

biological source

mouse

biological source

mouse

biological source

rabbit

biological source

rabbit

technique(s)

immunocytochemistry: suitable, western blot: suitable, immunoprecipitation (IP): suitable

technique(s)

immunoprecipitation (IP): suitable, western blot: suitable

technique(s)

western blot: suitable

technique(s)

immunoprecipitation (IP): suitable, western blot: suitable

Gene Information

human ... PPP2CA(5515)

Gene Information

human ... PPP2CA(5515)

Gene Information

human ... PPP2CA(5515)

Gene Information

human ... PPP2R1A(5518)

Analysis Note

Control
Positive Antigen Control: Catalog #12-301, non-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for minigels.
routinely evaluated by immunoblot on RIPA lysates from A431 cells

Application

Anti-PP2A Antibody, C subunit, demethylated, clone 4b7 is an antibody against PP2A for use in IC, IP & WB.
Research Category
Signaling

Neuroscience
Research Sub Category
Kinases & Phosphatases

Neurodegenerative Diseases

Biochem/physiol Actions

unmethylated PP2A, C subunit

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

36kDa

Immunogen

peptide (C-RGEPHVTRRTPDYFL) corresponding to amino acids 295-309 of human PP2A, C subunit

Physical form

0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide before the addition of glycerol to 30%
Ascites

Preparation Note

2 years at -20°C

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

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Skladovací třída

10 - Combustible liquids

wgk

WGK 1


Osvědčení o analýze (COA)

Vyhledejte osvědčení Osvědčení o analýze (COA) zadáním čísla šarže/dávky těchto produktů. Čísla šarže a dávky lze nalézt na štítku produktu za slovy „Lot“ nebo „Batch“.

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Xiaoyu Xia et al.
Cell cycle (Georgetown, Tex.), 14(12), 1938-1947 (2015-04-04)
Leucine carboxyl methyltransferase-1 (LCMT1) and protein phosphatase methylesterase-1 (PME-1) are essential enzymes that regulate the methylation of the protein phosphatase 2A catalytic subunit (PP2AC). LCMT1 and PME-1 have been linked to the regulation of cell growth and proliferation, but the
Juyeon Hwang et al.
The Journal of biological chemistry, 291(40), 21008-21019 (2016-08-11)
The protein phosphatase 2A (PP2A) subfamily of phosphatases, PP2A, PP4, and PP6, are multifunctional serine/threonine protein phosphatases involved in many cellular processes. Carboxyl methylation of the PP2A catalytic subunit (PP2Ac) C-terminal leucine is regulated by the opposing activities of leucine
Vitali Stanevich et al.
Molecular cell, 41(3), 331-342 (2011-02-05)
Proper formation of protein phosphatase 2A (PP2A) holoenzymes is essential for the fitness of all eukaryotic cells. Carboxyl methylation of the PP2A catalytic subunit plays a critical role in regulating holoenzyme assembly; methylation is catalyzed by PP2A-specific methyltransferase LCMT-1, an
Ying Zhou et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 34(12), 16414-16431 (2020-10-19)
Polyphyllin I (PPI) is a natural phytochemical drug isolated from plants which can inhibit the proliferation of cancer cells. One of the PPI tumor-inhibitory effects is through downregulating the expression of Cancerous Inhibitor of PP2A (CIP2A), the latter, is found
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