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05-484

Sigma-Aldrich

Anti-EGFR (non-phospho-Tyr1173) Antibody, clone 20G3

clone 20G3, Upstate®, from mouse

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

100

antibody form

purified antibody

antibody product type

primary antibodies

clone

20G3, monoclonal

species reactivity

human, mouse

manufacturer/tradename

Upstate®

technique(s)

immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG1κ

NCBI accession no.

NM_005228

UniProt accession no.

P00533

shipped in

wet ice

target post-translational modification

phosphorylation (non-phospho-Tyr1173)

Gene Information

human ... EGFR(1956)
mouse ... Egfr(13649)

Specificity

The motif NAEYLR of the EGF-R (unphosphorylated at Tyr1173), this antibody does not interact with the phosphorylated (pTyr1173) form of the EGF receptor. Tyr1173 corresponds to Tyr1197 in the human (isoform a) and mouse precursor proteins, and to Tyr1196 in the mature rat and pig precursor proteins.

Immunogen

peptide containing the unphosphorylated Tyr1173 motif (NAE[Y]LR) of the human EGF receptor

Application

Detect EGFR (non-phospho-Tyr1173) using this Anti-EGFR (non-phospho-Tyr1173) Antibody, clone 20G3 validated for use in IP & WB.
Research Category
Signaling
Research Sub Category
Growth Factors & Receptors

Quality

routinely evaluated by immunoblot on RIPA lysates from unstimulated HFF cells

Target description

175kDa

Linkage

Replaces: 04-337; 04-341

Physical form

100µg of lyophilized thiophilic and size exclusion chromatography purified mouse IgG1 from 1mL 2x PBS / 0.09 % Na-azide / PEG and Sucrose. Reconstitute with 100 µL H2O (15 min, RT) for a final concentration of 1 mg/mL.
Format: Purified
Thiophilic adsoption and size exclusion chromatography

Storage and Stability

2 years at -20°C

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

10 - Combustible liquids

wgk_germany

WGK 1

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Gang Chen et al.
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Alteration of epidermal growth factor receptor activity by mutation of its primary carboxyl-terminal site of tyrosine self-phosphorylation.
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The Journal of Biological Chemistry, 263, 3610-3617 (1988)
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The Journal of Biological Chemistry, 267, 8672-8678 (1992)
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Siggs, OM; Grieve, A; Xu, H; Bambrough, P; Christova, Y; Freeman, M
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