T7009

Thrombin from human plasma

lyophilized powder, ≥1,000 NIH units/mg protein (E1%/280, 18.3)

• Synonym(s): Factor IIa
• CAS Number: 9002-04-4
• Enzyme Commission number: 3.4.21.5 (BRENDA, IUBMB)
• EC Number: 232-648-7
• MDL number: MFCD00082072
• UNSPSC Code: 12352204
• NACRES: NA.54

Properties

• form: lyophilized powder
• Quality Level: 200
• specific activity: ≥1,000 NIH units/mg protein (E1%/280, 18.3)
• mol wt: 37.4 kDa
• impurities: HIV and HBsAg, source material tested negative
• UniProt accession no.: P00734
• application(s): diagnostic assay manufacturing
• storage temp.: −20°C
• Gene Information: human ... F2(2147)

Description

General description

The F2 gene encodes thrombin protein and is mapped to human chromosome 11p11.2.

Application

Thrombin from human plasma has been:

• dissolved in CaC_l2 and added to poly ethylene glycolated fibrinogen/cell solution for fibrin production
• to polymerize fibrin on well plates
• used as an endogenous agonist proteinase for proteinase-activated receptors : PAR1, PAR3 and PAR4 for stimulation of cells

Biochem/physiol Actions

Thrombin is an endolytic serine protease which stimulates vascular damage induced-leukocytes, platelets, endothelial and mesenchymal cell production. This action is mediated via G-protein-coupled receptor. It catalysis the cleavage of fibrinogen at Arg−Gly bonds to form fibrin and release fibrinopeptides A and B. The predominant form of thrombin in vivo is the zymogen prothrombin (factor II), which is produced in the liver. Prothrombin is a coagulation activation factor, high levels of which indicates cancer. Thrombin can be used to cleave fusion proteins. Cleavage of fusion proteins can be carried out at a thrombin:fusion protein ratio of 1:500.

Serine protease that selectively cleaves Arg-Gly bonds in fibrinogen to form fibrin and fibrinopeptides A and B.

Unit Definition

Activity is expressed in NIH units obtained by direct comparison to a NIH Thrombin Reference Standard

Physical form

Lyophilized from saline sodium citrate buffer, pH 6.5

Analysis Note

The NIH assay procedure uses 0.2 ml diluted plasma (1:1 with saline) as a substrate and 0.1ml of thrombin sample (stabilized in a 1% buffered albumin solution) based on a modification of the method of Biggs. Only clotting times in the range of 15-25 seconds are used for determining thrombin concentrations.

Other Notes

View more information on thrombin at www.sigma-aldrich.com/enzymeexplorer.

Disclaimer

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Safety Information

• Pictograms: GHS08
• Signal Word: Danger
• Hazard Statements: H334
• Precautionary Statements: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable