SRP6147

Ubiquitin Activating Enzyme E1 human

≥95% (SDS-PAGE)

• Synonym(s): AMCX1, POC20, UBA1, UBE1, Ubiquitin E1
• UNSPSC Code: 12352200
• NACRES: NA.32

Properties

• biological source: human
• Assay: ≥95% (SDS-PAGE)
• form: liquid
• potency: >3 nM
• mol wt: 118
• packaging: pkg of 50 μg
• NCBI accession no.: P22314
• shipped in: dry ice
• storage temp.: −70°C
• Gene Information: human ... UBA1(7317)

Description

General description

Research area: Cell Signaling

Ubiquitin activating enzyme 1 (UBA1) or UBE1, is an ubiquitin-activating enzyme. The gene encoding it is localized on human chromosome Xp11.23.[1][2]

Application

Ubiquitin Activating Enzyme E1 human has been used in in vitro and in vivo ubiquitination assays.[3]

Biochem/physiol Actions

Ubiquitin activating enzyme 1 (UBA1) has a role in cellular protein homeostasis. It is the first enzyme utilized in the pathway to conjugate ubiquitin onto target substrates through an ubiquitin-protein isopeptide bond. The C-terminal glycine of an ubiquitin (G76) is initially adenylated in an ATP dependent manner activating it so it can be transferred to the active site cysteine of UBE1 forming an ubiquitin-UBE1 thiolester. With the help of the other enzymes in the ubiquitin conjugation pathway (E2/E3 enzymes) the ubiquitin is transferred to a specific lysine on a target substrate. The protein has been associated with spinal muscular atrophy (SMA) and Huntington′s disease (HD).[1][4][5]

Physical form

In 50 mM Tris (pH 8.0), 100 mM NaCl and 10% glycerol.

Preparation Note

Centrifuge the vial prior to opening.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable