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Merck

SRE0062

Sigma-Aldrich

Tris-Borate EDTA Buffer, 10X Concentrate

Synonym(s):

TBE Buffer

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About This Item

UNSPSC Code:
41105319
NACRES:
NA.25

Pricing and availability is not currently available.

form

liquid

Quality Level

application(s)

diagnostic assay manufacturing

storage temp.

room temp

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This Item
394238658847684457
assay

97%

assay

≥99.5%

assay

97% (HPLC)

assay

>99.9%

solubility

water, pH >9: soluble

solubility

water: miscible

solubility

-

solubility

chlorobenzene: soluble, organic solvents: soluble, toluene: soluble

mp

>300 °C

mp

-

mp

-

mp

-

Application

Tris-Borate EDTA (TBE) buffer is commonly used in the electrophoresis of nucleic acids in agarose and polyacrylamide gels.

TBE buffer is recommended for resolution of RNA and DNA fragments smaller than 1500 bp.

Physical form

10X TBE buffer stock solution contains 0.89 M Trizma(R) with 0.02 M EDTA adjusted to pH 8.3 with boric acid. Dilute with ultrapure water to 1X.

Notes: If precipitation is present in the 10X concentrated buffer, warm the bottle to 37 °C and mix until completely dissolved prior to dilution.
It is recommended 1X working solutions be filtered through a 0.2 mm filter before use.

Pictograms

Health hazard

Signal Word

Danger

Hazard Statements

Hazard Classifications

Repr. 1B

Storage Class Code

6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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