SAE0088
Filipin complex ready made solution
from Streptomyces filipinensis, 5mg/ml in DMSO based solution
Synonym(s):
Filipin solution
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About This Item
UNSPSC Code:
12352200
NACRES:
NA.77
Pricing and availability is not currently available.
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Assay
≥90% (HPLC)
form
ready-to-use solution
storage condition
protect from light
shipped in
dry ice
storage temp.
−20°C
General description
Filipin Complex, the collective name given to family of isomeric polyene macrolides isolated from cultures of S. filipinensis, with Filipin III being the majority part of the complex.[1]
Application
Filipin complex readymade solution has been used in the staining of HeLa cells for cytoskeletal imaging.[2]
Filipin has been used in a double staining procedure as a probe for the detection of lipoproteins in polyacrylamide gel and immobilized on nitrocellulose membranes. It is also widely used to localize and quantitate unesterified cholesterol by virtue of a specific fluorescent complex.
Biochem/physiol Actions
Filipin is an antibiotic with antifungal functionality.[3] Filipin complex has been found to alter cell membrane structure by interacting with the membrane sterols ergosterols and cholesterol.[1] On binding to cholesterol, filipin shows fluorescence shift. As such, filipin complex has become a useful tool for diagnosis of Niemann-Pick type C disease, and for detection and quantification of cholesterol in cell membranes and lipid quantification in brain.[3]
Other Notes
Filipin complex ready made solution is very sensitive to air and light. Upon receipt aliquot and store at -20°C. Avoid freeze thaw cycles.
Filipin complex is used in various concentrations depending on the specific protocol used. Dilute 5mg/ml stock solution with appropriate buffer according to the used protocol.
Filipin complex interaction with cholesterol alters the absorption and fluorescence spectra, for visualization with a fluorescence microscope use excitation at 340-380 nm and emission at 385-470 nm (Filipin fluorescent staining photo-bleaches very rapidly, thus the sample should be analyzed immediately).
Filipin complex is used in various concentrations depending on the specific protocol used. Dilute 5mg/ml stock solution with appropriate buffer according to the used protocol.
Filipin complex interaction with cholesterol alters the absorption and fluorescence spectra, for visualization with a fluorescence microscope use excitation at 340-380 nm and emission at 385-470 nm (Filipin fluorescent staining photo-bleaches very rapidly, thus the sample should be analyzed immediately).
Storage Class Code
10 - Combustible liquids
WGK
WGK 2
Flash Point(F)
188.6 °F - closed cup
Flash Point(C)
87 °C - closed cup
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