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SAE0044

Sigma-Aldrich

Choline Oxidase from Arthrobacter sp.

recombinant, expressed in E. coli ≥12 units/mg protein

Synonym(s):

COD, COX, ChOx, Choline: oxygen 1-oxidoreductase

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About This Item

Enzyme Commission number:
UNSPSC Code:
12352204
NACRES:
NA.54

biological source

bacterial (arthrobacter sp.)

Quality Level

recombinant

expressed in E. coli ≥12 units/mg protein

form

powder

specific activity

≥12 units/mg protein

technique(s)

activity assay: suitable

UniProt accession no.

storage temp.

−20°C

General description

Research area: Cell Signaling

Choline oxidase from Arthrobacter sp. is a flavoprotein, which is a member of the glucose–methanol–choline (GMC). The codA gene encodes the choline oxidase enzyme.

Application

Choline oxidase from Arthrobacter sp. has been used in the enzymatic determination of choline in milk using a flow injection analysis (FIA) system with potentiometric detection. It has been used for fluorescent detection of choline released during autotaxin (ATX) activity in conditioned media and plasma.

Biochem/physiol Actions

Choline oxidase from Arthrobacter sp. is a flavoprotein, which is a member of the GMC-oxidoreductase family. The enzyme catalyzes the four-electron-oxidation of choline to glycine betaine via the intermediate betaine aldehyde, in two sequential FAD-dependent reaction steps. The enzyme is useful for enzymatic determination of phosphatidylcholine by coupling with phospholipase D or determination of sphingomyelin by coupling with sphingomyelinase and for cholinesterase activity assays.Choline oxidase (COD) mediating glycine betaine biosynthetic pathway plays a vital role in creating stress resistant transgenic plants.

Unit Definition

One unit will form 1 μmole of H2O2 with oxidation of 1 μmole of choline to betaine aldehyde per min at pH 8.0 at 37 °C. Note: During the conversion of choline to betaine by choline oxidase, 2 μmoles of H2O2 are produced for every μmole of choline.

Preparation Note

It is recommended to reconstitute choline oxidase in phosphate buffer at pH 7.5 supplemented with 10mM EDTA. Reconstituted material can be stored at 2-8 °C for at least 2 weeks.

Storage and Stability

Tightly closed.Dry. Keep locked up or in an area accessible only to qualified or authorized
persons

Pictograms

Health hazard

Signal Word

Danger

Hazard Statements

Precautionary Statements

Hazard Classifications

Resp. Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

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Rapid, specific, and sensitive measurements of plasma sphingomyelin and phosphatidylcholine.
Hojjati M.R., and Jiang X.C.
Journal of Lipid Research, 47, 673-676 (2006)
New enzymatic assay of cholinesterase activity
Okabe, H. et al.,
Clinica Chimica Acta; International Journal of Clinical Chemistry, 80, 87-94 (1977)
Transformation of Synechococcus with a gene for choline oxidase enhances tolerance to salt stress.
Deshnium P, et al.
Plant Molecular Biology, 29(5), 897-907 (1995)
Transformation of Arabidopsis thaliana with the codA gene for choline oxidase; accumulation of glycinebetaine and enhanced tolerance to salt and cold stress.
Hayashi H, et al.
The Plant Journal, 12(1), 133-142 (1997)
The use of bacterial choline oxidase, a glycinebetaine-synthesizing enzyme, to create stress-resistant transgenic plants
Plant Physiology, 180-188 (2001)

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