SAB4600176

Anti-Mouse IgG (H+L), highly cross-adsorbed, CF^™ 647 antibody produced in donkey

~2 mg/mL, affinity isolated antibody

• UNSPSC Code: 12352203
• NACRES: NA.46

Properties

• biological source: donkey
• Quality Level: 100
• conjugate: CF^™ 647 conjugate
• antibody form: affinity isolated antibody
• antibody product type: secondary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• species reactivity: mouse
• concentration: ~2 mg/mL
• technique(s): flow cytometry: 1-10 μg/mL; immunocytochemistry: suitable; immunohistochemistry: suitable; indirect immunofluorescence: 1-10 μg/mL
• fluorescence: λ_ex 650 nm; λ_em 665 nm
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified

Description

General description

Immunoglobulin G (IgG) is a glycoprotein antibody with two equivalent light chains and a pair of identical heavy chains. CF^™ 647 is an organic cyanine dye useful in fluorescent microscopy studies. Antibody–CF^™ 647 conjugates are affinity-purified antibodies labeled with a far-red fluorescent dye, CF^™ 647. CF^™ dyes are excellent alternatives to Alexa Fluor^™ dyes for antibody labeling with exceptional brightness, photostability, and/or specificity.

Specificity

Binds all mouse IgGs (minimal cross-reaction with bovine, chicken, goat, guinea pig, hamster, horse, human, rabbit and sheep serum proteins)

Immunogen

mouse IgG (H+L)

Biochem/physiol Actions

Immunoglobulin G (IgG) on pepsin digestion results in fragment crystallizable (Fc), which comprises the H chain constant region. Papain digestion of IgG results in the generation of fragment antigen-binding (Fab) comprising of one complete L chain and a variable and CH1 region of H chain.

Features and Benefits

Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Physical form

Supplied in phosphate buffered saline with 0.05% sodium azide, 50% glycerol and 2 mg/mL bovine serum albumin.

Preparation Note

Protect from light.

Legal Information

This product is distributed by Sigma-Aldrich Co. under the authorization of Biotium, Inc. This product is covered by one or more US patents and corresponding patent claims outside the US patents or pending applications owned or licensed by Biotium, Inc. including without limitation: 12/334,387; 12/607,915; 12/699,778; 12/850,578; 61/454,484. In consideration of the purchase price paid by the buyer, the buyer is hereby granted a limited, non-exclusive, non-transferable license to use only the purchased amount of the product solely for the buyer′s own internal research in a manner consistent with the accompanying product literature. Except as expressly granted herein, the sale of this product does not grant to or convey upon the buyer any license, expressly, by implication or estoppel, under any patent right or other intellectual property right of Biotium, Inc. Buyer shall not resell or transfer this product to any third party, or use the product for any commercial purposes, including without limitation, any diagnostic, therapeutic or prophylactic uses. This product is for research use only. Any other uses, including diagnostic uses, require a separate license from Biotium, Inc. For information on purchasing a license to use this product for purposes other than research, contact Biotium, Inc., 3159 Corporate Place, Hayward, CA 94545, Tel: (510) 265-1027. Fax: (510) 265-1352. Email: btinfo@biotium.com.

ALEXA FLUOR is a trademark of Life Technologies

CF is a trademark of Biotium, Inc.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable