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Sigma-Aldrich

Thrombin CleanCleave™ Kit

Synonym(s):

Thrombin Cleavage Kit

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About This Item

UNSPSC Code:
12352200
NACRES:
NA.56

form

suspension

Quality Level

200

mol wt

37 kDa

technique(s)

protein purification: suitable

shipped in

wet ice

storage temp.

2-8°C

General description

The Thrombin CleanCleave Kit contains a 50% suspension fo thrombin-agarose produced by immobilizing bovine thrombin and is designed for cleavage of recombinant fusion proteins

Application

For cleaving a tag from a recombinant fusion protein which contains the thrombin recognition sequence. Thrombin-agarose is compatible with recombinant proteins expressed in various types of expression systems.

Features and Benefits

  • Fast, efficient cleavage in as little as 2 hours
  • Thrombin is covalently bound to agarose for easy removal.
  • The robust cleavage reaction is effective at temperatures from 4 °C to 37 °C and over a wide range of pH and ionic strengths.
  • Cleave tags even in the presence of 0.1% Triton™, 1 M urea or 5 mM EDTA
  • Thrombin-agarose is reusable.

Quantity

200 μl of a 50% slurry of thrombin-agarose cleaves >85% of 1 mg of fusion protein.

Legal Information

CleanCleave is a trademark of Sigma-Aldrich Co. LLC
Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow

Kit Components Only

Product No.
Description
  • Thrombin Cleavage Buffer, 10× 10 mL

Storage Class Code

10 - Combustible liquids

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
0000395426
0000331150
0000327823
0000327828
0000395426

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Articles

Cleavage and Purification of GST-Tagged Protein Bound to GSTrap

This page shows how to cleave and purify GST-tagged proteins bound to GSTrap from Cytiva.

Protocols

Removal of GST Tag by Enzymatic Cleavage

This page how to remove GST tags by enzymatic cleavage using Cytiva products.

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