Skip to Content
Merck
All Photos(1)

Key Documents

View All Documentation

R6504

Sigma-Aldrich

RPMI-1640 Medium

With L-glutamine, without sodium bicarbonate, powder, suitable for cell culture

Synonym(s):

Roswell Park Memorial Institute 1640 medium

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

MDL number:
MFCD00217820
UNSPSC Code:
12352207
NACRES:
NA.75

Quality Level

500

form

powder

technique(s)

cell culture | mammalian: suitable

components

L-glutamine: yes
sodium pyruvate: no
HEPES: no
NaHCO3: no
phenol red: yes

shipped in

ambient

storage temp.

2-8°C

Looking for similar products? Visit Product Comparison Guide

General description

RPMI 1640 Medium was developed at Roswell Park Memorial Institute in 1966 by Moore and his co-workers. A modification of McCoy′s 5A Medium, it was formulated to support lymphoblastoid cells in suspension culture, but it has since been shown to support a wide variety of cells that are anchorage-dependent. Originally intended to be used with a serum supplement, RPMI 1640 has been shown to support several cell lines in the absence of serum. It has also been widely used in fusion protocols and in the growth of hybrid cells. This medium is suitable for culturing human normal and neoplastic leukocytes.
Roswell Park Memorial Institute medium is commonly known as RPMI. It is a form of medium used in cell culture and tissue culture. This medium contains phosphate and is formulated for use in a 5% carbon dioxide atmosphere. It is used for the growth of human lymphoid cells.

Application

RPMI-1640 Medium is used for the following applications:
  • used in cell culture medium (i.e. Murine thymoma EL-4 cells were cultured in RPMI 1640 medium supplemented with other components)
  • used for the mycobacterial infection of splenocytes (in antibiotic-free RPMI 1640 medium)
  • used for cell isolation and culture
  • used in culture medium (as one of the component) during lymphocyte separation and culture, ELISPOT Assay, Ex Vivo proliferation assay
  • used in medium for gp39 gene expression and CD40-Immunoglobuin binding assays
  • used for the preparation of antifungal agents

Quantity

Formulated to contain 10.4 grams of powder per liter of medium.

Reconstitution

Supplement with 2.0 g/L sodium bicarbonate.

Storage Class Code

11 - Combustible Solids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Sook Yee Boo et al.
Scientific reports, 10(1), 18348-18348 (2020-10-29)
The infectious bursal disease (IBD) is an acute immunosuppressive viral disease that significantly affects the economics of the poultry industry. The IBD virus (IBDV) was known to infect B lymphocytes and activate macrophage and T lymphocytes, but there are limited
Hui Tian et al.
Analytical chemistry, 88(23), 11384-11389 (2016-11-02)
MicroRNA (miRNA) analysis in a single cell is extremely important because it allows deep understanding of the exact correlation between the miRNAs and cell functions. Herein, we wish to report a highly sensitive and precisely quantitative assay for miRNA detection
M E Klepser et al.
Antimicrobial agents and chemotherapy, 41(6), 1392-1395 (1997-06-01)
Time-kill curves were determined for three isolates of Candida albicans tested against fluconazole and amphotericin B at multiples of the MIC. Fluconazole produced fungistatic activity, with concentration-related growth effects observed over a narrow range of concentrations. Amphotericin B exhibited fungicidal
Manuel Cuenca-Estrella et al.
Antimicrobial agents and chemotherapy, 46(11), 3644-3647 (2002-10-18)
A two-laboratory study was performed to evaluate the correlation between the NCCLS M27-A and EUCAST microdilution procedures for antifungal testing of Candida spp. A panel of 109 bloodstream isolates was tested against amphotericin B, flucytosine, fluconazole, and itraconazole. Overall, the
M Farrington et al.
Proceedings of the National Academy of Sciences of the United States of America, 91(3), 1099-1103 (1994-02-01)
Common variable immunodeficiency (CVI) is characterized by hypogammaglobulinemia and recurrent bacterial infections due to failure of CVI B cells to differentiate in vivo into immunoglobulin-secreting plasma cells. We hypothesized that T-cell dysfunction resulting in abnormal contact-mediated B-cell activation may play
View All Related Papers

Articles

ELISpot Assay: Functional Cellular Immunology

ELISpot assay provides qualitative and quantitative information on immune responses, visualizing multiple secretory products from single responding cells.

ELISpot Assay: Functional Cellular Immunology

ELISpot assay provides qualitative and quantitative information on immune responses, visualizing multiple secretory products from single responding cells.

ELISpot Assay: Functional Cellular Immunology

ELISpot assay provides qualitative and quantitative information on immune responses, visualizing multiple secretory products from single responding cells.

ELISpot Assay: Functional Cellular Immunology

ELISpot assay provides qualitative and quantitative information on immune responses, visualizing multiple secretory products from single responding cells.

Protocols

Powdered Media Preparation Instructions

Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service