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Key Documents

Q1126

Sigma-Aldrich

Q Sepharose Fast Flow

preswollen, 45-165 μm (wet), exclusion limit ~4,000,000 Da

Synonym(s):

Q Sepharose

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About This Item

MDL number:
UNSPSC Code:
47101511
NACRES:
NA.56

form

preswollen

Quality Level

technique(s)

RNA extraction: suitable

particle size

45-165 μm (wet)

pore size

~4,000,000 Da exclusion limit

capacity

0.18-0.24 mmol Cl-/mL, packed gel

storage temp.

2-8°C

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General description

−Ο−CH2CHOHCH2OCH2CHOHCH2N+(CH3)3

Application

Q Sepharose is used in protein chromatography, ion exchange chromatography and anion exchange media. Q Sepharose has been used to study compounds of plant defense with applications for natural control of phytopathogenic fungi. Q Sepharosehas also been used to develop an efficient method for extracting high-quality mRNA from soil and to study the immunomodulatory proteins from garlic (Allium sativum).

Physical form

Preswollen in 20% ethanol

Legal Information

Sepharose is a trademark of Cytiva

Pictograms

Flame

Signal Word

Warning

Hazard Statements

Hazard Classifications

Flam. Liq. 3

Storage Class Code

3 - Flammable liquids

WGK

WGK 1

Flash Point(F)

100.4 - 109.4 °F - closed cup

Flash Point(C)

38 - 43 °C - closed cup

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Shikimate dehydrogenase from pepper (<I>Capsicum annuum</I>) seedlings. Purification and properties.
Diaz, J., and Merino, F.
Physiologia Plantarum, 100(1), 147-152 (1997)
Irini Bournazou et al.
The Journal of clinical investigation, 119(1), 20-32 (2008-11-27)
Apoptosis is a noninflammatory, programmed form of cell death. One mechanism underlying the non-phlogistic nature of the apoptosis program is the swift phagocytosis of the dying cells. How apoptotic cells attract mononuclear phagocytes and not granulocytes, the professional phagocytes that
E G Campos et al.
International journal for parasitology, 29(5), 655-662 (1999-07-15)
Cytochrome c peroxidase oxidises hydrogen peroxide using cytochrome c as the electron donor. This enzyme is found in yeast and bacteria and has been also described in the trematodes Fasciola hepatica and Schistosoma mansoni. Using partially purified cytochrome c peroxidase
B Oster et al.
The Journal of general virology, 89(Pt 1), 87-96 (2007-12-20)
Here, we demonstrate that human herpesvirus 6B (HHV-6B) infection upregulates the tumour suppressor p53 and induces phosphorylation of p53 at Ser392. Interestingly, phosphorylation at the equivalent site has previously been shown to correlate with p53 tumour suppression in murine models.
Suzana M Ribeiro et al.
Peptides, 32(5), 868-874 (2010-10-20)
Antifungal proteins and peptides, essential compounds for plant defense, have been isolated from several tissues of various plants. These proteins could be used as a natural alternative to control phytopathogenic fungi. In this report a heterodimeric antifungal protein named Pa-AFP1

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