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PEROX1

Sigma-Aldrich

Peroxisome Isolation Kit

isolate peroxisomes from tissues and cells

Synonym(s):

Isolation Kit for Peroxisomes, Kit for Peroxisomes, Peroxisome Kit

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About This Item

UNSPSC Code:
12352200
NACRES:
NA.32

Quality Level

200

technique(s)

centrifugation: suitable
fractionation: suitable

shipped in

wet ice

storage temp.

2-8°C

Related Categories

General description

Isolated peroxisomes are used for studying lipid β-oxidation, amino acid metabolism and biosynthesis of ether-linked glycerolipids and bile acids.

Application

The Perixosome Isolation Kit provides all the necessary reagents and a detailed protocol for the isolation of highly purified peroxisomes from animal tissues and cells, by differential density gradient centrifugation using iodixanol [OptiPrep™]. This kit has been used for preparation of peroxisomes from rat liver, rat kidney and rabbit liver as well as HEK293 and HepG2 cells.

Features and Benefits

  • Specially formulated extraction reagents for research scale applications - save time and minimize waste
  • Produces functional intact organelles - resulting peroxisomes are suitable for functional studies, metabolic assays, protein profiling, and disease state analysis
  • Compatible with products for structure confirmation - easily confirm intactness with companion test kit, Cytochrome C Reductase Assay Kit (Cat. No. CY0100)

Other Notes

Upon receiving the kit, the Protease Inhibitor Cocktail (Product Code P 8340) should be stored at –20 °C and the OptiPrep Density Gradient Medium (Product Code O3028) should be stored at room temperature.

Legal Information

OptiPrep is a trademark of Serumwerk Bernburg AG

Kit Components Also Available Separately

Product No.
Description
SDS
  • P8340Protease Inhibitor Cocktail, for use with mammalian cell and tissue extracts, DMSO solutionSDS

Pictograms

Corrosion
GHS05

Signal Word

Warning

Hazard Statements

H290,H315,H319

Hazard Classifications

Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2

Storage Class Code

8A - Combustible corrosive hazardous materials

Flash Point(F)

188.6 °F - closed cup

Flash Point(C)

87 °C - closed cup

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Shurong Hou et al.
SLAS discovery : advancing life sciences R & D, 22(7), 887-896 (2017-03-28)
Primary hyperoxaluria is the underlying cause of oxalosis and is a life-threatening autosomal recessive disease, for which treatment may require dialysis or dual liver-kidney transplantation. The most common primary hyperoxaluria type 1 (PH1) is caused by genetic mutations of a
C M Rodrigues et al.
Journal of lipid research, 37(3), 540-550 (1996-03-01)
We recently demonstrated that the formation of delta 22-bile acids is a quantitatively major pathway for normal bile acid synthesis in the adult male Sprague-Dawley rat. This pathway is specific for 7 beta-hydroxy bile acids and, when ursodeoxycholic acid is
P B Lazarow et al.
Proceedings of the National Academy of Sciences of the United States of America, 73(6), 2043-2046 (1976-06-01)
Purified rat liver peroxisomes contain a cyanide-insensitive fatty acyl-CoA oxidizing system that uses O2 and NAD as electron acceptors. The system was detected by the ability of added palmitoyl-CoA to elicit O2 consumption, H2O2 production, and O2-dependent NAD reduction. The
M Une et al.
Journal of lipid research, 37(12), 2550-2556 (1996-12-01)
The oxidation of the side chains of two potential bile acid intermediates, 3 alpha,7 alpha,12 alpha-trihydroxy-5 beta-cholestanoic acid (THCA) and 3 alpha,7 alpha-dihydroxy-5 beta-cholestanoic acid (DHCA), were investigated in rat liver mitochondria and peroxisomes. Both THCA and DHCA were efficiently
G P Mannaerts et al.
Biochimie, 75(3-4), 147-158 (1993-01-01)
This article summarizes our current knowledge of the metabolic pathways present in mammalian peroxisomes. Emphasis is placed on those aspects that are not covered by other articles in this issue: peroxisomal enzyme content and topology; the peroxisomal beta-oxidation system; substrates
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Organelle Isolation

Centrifugation separates organelles based on size, shape, and density, facilitating subcellular fractionation across various samples.

Organelle Isolation

Centrifugation separates organelles based on size, shape, and density, facilitating subcellular fractionation across various samples.

Organelle Isolation

Centrifugation separates organelles based on size, shape, and density, facilitating subcellular fractionation across various samples.

Organelle Isolation

Centrifugation separates organelles based on size, shape, and density, facilitating subcellular fractionation across various samples.

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