Skip to Content
Merck
All Photos(1)

Key Documents

MCL1

Sigma-Aldrich

Mammalian Cell Lysis Kit

Synonym(s):

Cell Lysis Reagent

Sign Into View Organizational & Contract Pricing


About This Item

UNSPSC Code:
12352200
NACRES:
NA.56

usage

 kit sufficient for 250 extractions (100 mm tissue culture plates)

Quality Level

technique(s)

immunoprecipitation (IP): suitable

shipped in

dry ice

storage temp.

−20°C

Application

The product contains all required reagents for the disintegration of mammalian cells and tissue for subsequent analysis of protein targets. Cellular proteins are stabilized during lysis and are suitable for immunoassays, immunoprecipitation, affinity purification and other functional assays.
Mammalian cell lysis kit was used to test the role of vascular endothelial growth factor (VEGF) receptor-1 and its ligand VEGF-B in motor neuron degeneration.

Features and Benefits

  • The kit can be used to prepare cell lysis buffer and other modified buffers
  • The kit enables the researcher to change the buffer′s components to obtain maximum efficiency for a specific protein

Components

The mammalian cell lysis kit is composed of solutions to be mixed and used for lysis of adherent cells, non-adherent cells, and tissues. It contains buffer, detergents, NaCl and protease inhibitor cocktail solutions.

Kit Components Also Available Separately

Product No.
Description
SDS

  • P8340Protease inhibitor cocktail 2.5 mLSDS

Pictograms

CorrosionExclamation mark

Signal Word

Danger

Hazard Classifications

Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Skin Irrit. 2 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

10 - Combustible liquids

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Shinsuke Yasuda et al.
Journal of immunology (Baltimore, Md. : 1950), 179(7), 4890-4900 (2007-09-20)
Dysregulation of Ras guanyl nucleotide-releasing protein 1 (RasGRP1) in mice results in a systemic lupus erythematosus (SLE)-like disorder. We therefore looked for defective isoforms and/or diminished levels of human RasGRP1 in a cohort of SLE patients. PBMCs were collected from
Shintaro Nakao et al.
Blood, 117(3), 1081-1090 (2010-08-14)
Angio- and lymphangiogenesis are inherently related processes. However, how blood and lymphatic vessels regulate each other is unknown. This work introduces a novel mechanism explaining the temporal and spatial relation of blood and lymphatic vessels. Vascular endothelial growth factor-A (VEGF-A)
Shintaro Nakao et al.
Investigative ophthalmology & visual science, 54(6), 3830-3836 (2013-04-13)
Inflammation and immune cells regulate choroidal neovascularization (CNV) and could become therapeutic targets in age-related macular degeneration (AMD). Lymphangiogenesis is a key component of various inflammatory diseases. Whether lymphangiogenesis and lymph node-mediated immunity are involved in the pathogenesis of AMD
Lech Martyniszyn et al.
Viral immunology, 26(5), 322-335 (2013-10-15)
Several studies have provided evidence that complex relationships between autophagic and apoptotic cell death pathways occur in cancer and virus-infected cells. Previously, we demonstrated that infection of macrophages with Moscow strain of ectromelia virus (ECTV-MOS) induces apoptosis under in vitro
Shintaro Nakao et al.
The American journal of pathology, 178(4), 1913-1921 (2011-03-26)
Vascular adhesion protein-1 (VAP-1) contributes to inflammatory and angiogenic diseases, including cancer and age-related macular degeneration. It is expressed in blood vessels and contributes to inflammatory leukocyte recruitment. The cytokines IL-1β and vascular endothelial growth factor A (VEGF-A) modulate angiogenesis

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service