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MAK178

Sigma-Aldrich

Enolase Activity Assay Kit

Sufficient for 100 Colorimetric or Fluorometric tests

Synonym(s):

Enolase Detection Kit

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About This Item

UNSPSC Code:
12161503
NACRES:
NA.84

detection method

colorimetric
fluorometric

relevant disease(s)

immunological diseases; cardiovascular diseases

storage temp.

−20°C

Gene Information

human ... EBI4(387712) , ENO1(2023) , ENO2(2026)
mouse ... EBI4(226265) , ENO1(13806) , ENO2(13807)
rat ... EBI4(292138) , ENO1(24333) , ENO2(24334)

Related Categories

General description

Enolase is a multifunctional glycolytic enzyme that catalyzes the conversion of D-2-phosphoglycerate to phospho(enol)pyruvate (PEP) and water. It can function as a plasminogen receptor in endothelial, epithelial, and hematopoietic cells and hence, may be involved in fibrinolytic and intravascular systems. It is also known to act as a heat shock protein, which may have implications in transcriptional and pathological functions. Enolase has been implicated in autoimmune and systemic diseases. Furthermore; serum neuron-specific enolase is known to function as a predictor of patient outcome post cardiac arrest. Thus enolase assays can be used for studying cellular functions like carbohydrate metabolism, transcription and other pathophysiological processes.

Application

Enolase Activity Assay Kit has been used to measure enolase activity.

Suitability

Suitable for the detection of enolase activity in biological samples.

Principle

The Enolase activity is determined by a coupled enzyme assay in which D-2-phosphoglycerate is converted to PEP, resulting in the formation of an intermediate that reacts with a peroxidase substrate, generating a colorimetric (570 nm) or fluorometric (λex = 535/λem = 587 nm) product proportional to the enolase activity present. One milliunit of enolase is the amount of enzyme that will generate 1.0 nmole of H2O2 per minute at pH 7.2 at 25 °C.

Kit Components Only

Product No.
Description
  • Enolase Assay Buffer
  • Peroxidase Substrate, in DMSO
  • Enolase Substrate Mix
  • Enolase Converter
  • Enolase Developer
  • Enolase Positive Control
  • Hydrogen Peroxide Standard, 0.88 M

Hazard Statements

H412

Precautionary Statements

P273 - P501

Hazard Classifications

Aquatic Chronic 3

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

EU REACH Annex XIV (Authorisation List)

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Global Profiling of Lysine Acetylation in Borrelia burgdorferi B31 Reveals Its Role in Central Metabolism.
Bontemps-Gallo S, et al.
Frontiers in Microbiology, 9, 2036-2036 (2018)
WW domain-binding protein 2 acts as an oncogene by modulating the activity of the glycolytic enzyme ENO1 in glioma.
Chen S, et al.
Cell Death & Disease, 9(3), 347-347 (2018)
Sébastien Bontemps-Gallo et al.
Frontiers in microbiology, 9, 2036-2036 (2018-09-21)
The post-translational modification of proteins has been shown to be extremely important in prokaryotes. Using a highly sensitive mass spectrometry-based proteomics approach, we have characterized the acetylome of B. burgdorferi. As previously reported for other bacteria, a relatively low number
Miriam Hippner et al.
Diagnostics (Basel, Switzerland), 12(2) (2022-02-26)
Alpha-enolase (ENO1) is a glycolytic metalloenzyme, and its overexpression occurs in numerous cancers, contributing to cancer cell survival, proliferation, and maintenance of the Warburg effect. Patients with an overexpression of ENO1 have a poor prognosis. The aim of the present
Xing Huang et al.
Autophagy, 15(7), 1258-1279 (2019-02-23)
Notwithstanding the numerous drugs available for liver cancer, emerging evidence suggests that chemotherapeutic resistance is a significant issue. HGF and its receptor MET play critical roles in liver carcinogenesis and metastasis, mainly dependent on the activity of receptor tyrosine kinase.

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