M2442
Anti-phospho-N-Methyl D-Aspartate NR2B Subunit (pTyr1472) antibody produced in rabbit
affinity isolated antibody, buffered aqueous solution
Synonym(s):
Anti-phospho-NMDA NR2B (pTyr1472)
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About This Item
Recommended Products
biological source
rabbit
Quality Level
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
mol wt
antigen 180 kDa
species reactivity
mouse, rat, Xenopus, chicken, human
technique(s)
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
indirect ELISA: suitable
western blot: 1:1,000 using rat brain (hippocampal) homogenate
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
phosphorylation (pTyr1472)
Gene Information
human ... GRIN2B(2904)
mouse ... Grin2b(14812)
rat ... Grin2b(24410)
General description
Rabbit polyclonal anti-phospho- N-Methyl D-Aspartate NR2B Subunit (pTyr-1472) antibody specifically recognizes rat receptor NR2B subunit (~180 kDa) phosphorylated on tyrosine 1472. It does not crossreact with non-phosphorylated NMDA NR2B. It is likely to detect human, mouse, chicken and Xenopus NMDA NR2B based on sequence homology.
Specifically recognizes the NMDA receptor NR2B subunit phosphorylated on tyrosine 1472. Does not cross-react with non-phosphorylated NMDAR. Two additional lower molecular weight immunoreactive bands are present in immunoblots of rat brain.
The ionotropic glutamate receptor is a prominent ligand-gated and voltage-gated ion channel in excitatory synaptic transmission in the mammalian central nervous system. The glutamate receptor was named the NMDA receptor for its agonist, N-methyl-D-aspartate. The NMDA channel is regulated by its phosphorylation. Functional NMDA receptors contain an active invariant subunit (NMDAR1) associated with one of several variable regulatory subunits. NR2B is one of the variant regulatory subunits associated with the active subunit NMDAR1 and localized primarily to the hippocampus and forebrain. Tyrosine phosphorylation sites on NR2B have been identified. Phosphorylation of tyrosine 1472 on the NR2B subunit is largely dependent on Fyn, a member of the tyrosine kinase oncogene family which regulates long-term potentiation, spatial learning, and hippocampal development, and is elevated upon the long-term potentiation (LTP) induction of hippocampal CA1 neurons. Tyrosine 1472 phosphorylation of NR2B is important for synaptic plasticity.
Immunogen
synthetic phosphorylated peptide derived from the region of NMDA receptor NR2B that is phosphorylated on tyrosine 1472.
Application
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)
Western Blotting (1 paper)
Rabbit polyclonal anti-phospho-N-Methyl D-Aspartate NR2B Subunit (pTyr-1472) antibody is used to tag the pTyr1472-NR2B subunit of NMDA for detection and quantitation by immunocytochemical and immunohistochemical (IHC) techniques. It is used as a probe to determine the presence and roles of pTyr1472 phosphorylated NR2B subunit of NMDA in neural functions such as long-term potentiation (LTP) and synaptic plasticity.
Physical form
Solution in 10 mM HEPES, pH 7.5, and 150 mM NaCl containing 100 μg/ml BSA and 50% glycerol.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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Zi-Fan Zhuang et al.
Frontiers in cellular neuroscience, 16, 981190-981190 (2022-10-04)
Trigeminal neuralgia (TN) is a peripheral nerve disorder often accompanied by abnormalities in mood. The lateral habenula (LHb) plays important roles in the modulation of pain and emotion. In the present study, we investigated the involvement of the LHb in
Sílvia Castany et al.
Scientific reports, 8(1), 3873-3873 (2018-03-03)
Sigma-1 receptor (σ1R) knockout (KO) CD1 mice, generated by homologous recombination, and separate pharmacological studies in wild type (WT) mice were done to investigate the role of this receptor in the development of pain-related behaviours (thermal hyperalgesia and mechanical allodynia)
Agnieszka Zelek-Molik et al.
Biomolecules, 11(2) (2021-03-07)
This study demonstrates how exposure to psychosocial crowding stress (CS) for 3, 7, and 14 days affects glutamate synapse functioning and signal transduction in the frontal cortex (FC) of rats. CS effects on synaptic activity were evaluated in FC slices
Sílvia Castany et al.
Frontiers in pharmacology, 10, 222-222 (2019-04-11)
Up to two-thirds of patients affected by spinal cord injury (SCI) develop central neuropathic pain (CNP), which has a high impact on their quality of life. Most of the patients are largely refractory to current treatments, and new pharmacological strategies
Bok Soon Go et al.
European neuropsychopharmacology : the journal of the European College of Neuropsychopharmacology, 26(12), 1989-1999 (2016-10-22)
Cocaine self-administration induces dysfunctional neuroadaptations in the prefrontal cortex that underlie relapse to cocaine-seeking. Cocaine self-administration disturbs glutamatergic transmission in the nucleus accumbens that is prevented by infusion of brain-derived neurotrophic factor (BDNF) into the prelimbic area of the prefrontal
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