L6170
β-Lactamase
recombinant, expressed in E. coli
Synonym(s):
Carbapenemase, Cephalosprinase
Sign Into View Organizational & Contract Pricing
All Photos(1)
About This Item
Recommended Products
recombinant
expressed in E. coli
Quality Level
form
powder
specific activity
≥20 U/mg (with cephalosporin C)
≥400 U/mg (with benzylpenicilin)
storage temp.
2-8°C
General description
β-Lactamase produced by bacteria is closely related to the penicillin binding proteins. β-Lactamase hydrolyzes β-lactum antibiotics and is the prime cause of resistance development by bacteria. There are four subclasses of β-Lactamases. Classes A, C and D form an acyl-enzyme via active site serine residue. Class B β-lactamases are metalloenzymes with zinc ion at their active site for β-lactam hydrolysis. Mutations in the β-lactamases has resulted in the generation of extended-spectrum β-lactamases (ESBLs). As close to 900 types of β-Lactamases are produced by microbes.
Application
β--lactamase is used to inactivate β-lactam antibiotics by breaking open the β-lactam ring. β--lactamase is used to study antibiotic resistance and resistance suppression. Product L6170 is recombinantly produced based on the sequence of the enzyme from Pseudomonas aeruginosa, and is expressed in E. coli.
β-Lactamase has been used in coumarin-cephalosporin-fluorescein-acetoxymethyl (CCF4)- β-Lactamase assay in dendritic cells and in the hydrolysis of meropenem (H-Mer) to generate 18O-labeled H-Mer.
Biochem/physiol Actions
β--lactamase inactivates β-lactam antibiotics by breaking open the β-lactam ring. Typical analysis for other substrates: ceftriaxon 2-4 un/mg; cefazolin 2-4 un/mg; ceftazidime 1.5-3 un/mg; cefoxitin 0.35-0.7 un/mg; cefepime 1.2-2.4 un/mg; cefuroxime 1.5-3 un/mg; cefotaxime 1-2 un/mg, oxacillin 4-8 un/mg; imipenem 1.2-2.4 un/mg; and meropenem 3-6 un/mg
Typical analysis for other substrates: ceftriaxon 2-4 un/mg; cefazolin 2-4 un/mg; ceftazidime 1.5-3 un/mg; cefoxitin 0.35-0.7 un/mg; cefepime 1.2-2.4 un/mg; cefuroxime 1.5-3 un/mg; cefotaxime 1-2 un/mg, oxacillin 4-8 un/mg; imipenem 1.2-2.4 un/mg; and meropenem 3-6 un/mg
Unit Definition
One unit will hydrolyze 1.0 μmole substrate per min (βI: benzylpenicillin; βII: cephalosporin C) in 50 mM phosphate buffer, pH 7.0 containing 10 μM zinc chloride at 25 ºC.
Physical form
Lyophilized powder containing sodium chloride and potassium phosphate.
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Customers Also Viewed
Updated functional classification of beta-lactamases
Antimicrobial Agents and Chemotherapy, 54(3), 969-976 (2010)
Determining carbapenemase activity with 18O labeling and targeted mass spectrometry
Analytical Chemistry, 85(22), 11014-11019 (2013)
Antibiotic resistance and extended spectrum beta-lactamases: Types, epidemiology and treatment
Saudi Journal of Biological Sciences, 22(1), 90-101 (2015)
VAMP8-mediated NOX2 recruitment to endosomes is necessary for antigen release
European Journal of Cell Biology, 96(7), 705-714 (2017)
ACS infectious diseases, 6(6), 1460-1479 (2020-04-25)
The formation of biofilms provides a formidable defense for many bacteria against antibiotics and host immune responses. As a consequence, biofilms are thought to be the root cause of most chronic infections, including those occurring on medical indwelling devices, endocarditis
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service