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L2158

Sigma-Aldrich

L-Leucine p-nitroanilide hydrochloride

chromogenic, ≥98% (HPLC), powder

Synonym(s):

H-Leu-pNA

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About This Item

Empirical Formula (Hill Notation):
C12H17N3O3 · HCl
CAS Number:
16010-98-3
Molecular Weight:
287.74
MDL number:
MFCD00058248
UNSPSC Code:
12352204
PubChem Substance ID:
24896304
NACRES:
NA.28

product name

L-Leucine p-nitroanilide hydrochloride, ≥98% (HPLC)

Quality Level

300

Assay

≥98% (HPLC)

form

powder

solubility

water: 25 mg/mL

storage temp.

2-8°C

SMILES string

Cl.CC(C)C[C@H](N)C(=O)Nc1ccc(cc1)N(=O)=O

InChI

1S/C12H17N3O3.ClH/c1-8(2)7-11(13)12(16)14-9-3-5-10(6-4-9)15(17)18;/h3-6,8,11H,7,13H2,1-2H3,(H,14,16);1H/t11-;/m0./s1

InChI key

PFOYXOWPGRWPLS-MERQFXBCSA-N

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Application

L-Leucine p-nitroanilide hydrochloride has been used as a substrate to measure the activity of leucine aminopeptidase (LAP):
  • for spectrophotometric assays
  • in the intestinal samples, such as duodenum, jejunum, and ileum
  • in the hemolymph of Manila clam

Substrates

Substrate for the colorimetric determination of leucine aminopeptidase.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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J Williams et al.
British poultry science, 49(3), 329-339 (2008-06-24)
1. The objective of this experiment was to study two feeding methods, which could potentially act on the gut microflora, the structure and/or the function of the digestive tract and thereby improve the performance of broilers. 2. Four dietary treatments
Characterization of proteolytic enzyme activities in macroalgae
Perez-Llorens JL, et al.
European Journal of Phycology, 38(1), 55-64 (2003)
Susan E Ford et al.
Fish & shellfish immunology, 23(2), 280-291 (2006-12-19)
Components of the haemolymph are understood to constitute the internal defense system of bivalve mollusks and their levels are often considered to be indicators of "health"; however, relatively little proof exists of the role that these elements play in the
Arun Kumar Singh et al.
International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases, 16(5), e375-e381 (2012-03-14)
Staphylococcus aureus has emerged as a major drug-resistant pathogen in hospital- and community-acquired infections. Leucine aminopeptidase (LAP) is known to be essential for survival of the bacteria; however the LAP of S. aureus has not been extensively characterized. In this
Arun Kumar Singh et al.
International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases, 16(5), e375-e381 (2012-03-14)
Staphylococcus aureus has emerged as a major drug-resistant pathogen in hospital- and community-acquired infections. Leucine aminopeptidase (LAP) is known to be essential for survival of the bacteria; however the LAP of S. aureus has not been extensively characterized. In this

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