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I3769

Sigma-Aldrich

Insulin-like Growth Factor-I human

≥95% (HPLC), recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

Synonym(s):

IGF, IGF-I, somatomedin c

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About This Item

MDL number:
MFCD00081716
UNSPSC Code:
12352202
NACRES:
NA.77

product name

Insulin-like Growth Factor-I human, IGF-I, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

biological source

human

Quality Level

200

recombinant

expressed in E. coli

Assay

≥95% (HPLC)

form

lyophilized powder

potency

0.1-10 ng/mL ED50/EC50

quality

endotoxin tested

mol wt

~7.6 kDa

packaging

pkg of 50 and 100 μg

technique(s)

cell culture | mammalian: suitable

impurities

≤1.00 EU/μg

UniProt accession no.

P05019

storage temp.

−20°C

Gene Information

human ... IGF1(3479) , Igf1(16000)

General description

Insulin-like growth factor I (IGF-I) is localized on human chromosome 12q23.2.

Application

Insulin-like Growth Factor-I human has been used:
  • in modified Dulbecco minimal essential medium for in vitro culture of testicular cells
  • in culture media, to study its effect on steroidogenesis during testicular development
  • in culture media for in vitro assay of splenic pro-erythroid cell growth and differentiation

Biochem/physiol Actions

IGF-I also known as somatomedin C, is secreted from the liver into circulation in a process regulated by pituitary growth hormone (GH) and so it mediates the growth-promoting activity of GH. In the developing embryo IGF-I is expressed primarily by mesenchymal-derived cells. After birth IGF-I expression in most extrahepatic tissues declines and hepatic expression of IGF-I becomes GH-regulated. Expression of IGF-I outside the liver is regulated differently, depending on the specific tissues. For example, gonadotropins and sex steroids regulate IGF-I expression within the reproductive system, while parathyroid hormone and sex steroids regulate IGF-I expression in bone. IGF-I is produced in several human tumors. IGF-I is mitogenic for a variety of cells including fibroblasts, osteoblasts, smooth muscle cells, fetal brain cells, neuroglial cells, and erythroid progenitor cells. IGF-I exerts its actions exclusively through the IGF-I receptor (IGF-IR). IGF-I induces endothelial cell migration and is involved in the regulation of angiogenesis.
Insulin-like growth factor I (IGF-I) plays a critical role in cellular energy metabolism and growth and development, especially prenatal growth.

Physical form

Lyophilized from 0.2 μm filtered solution in 50 mM acetic acid.

Reconstitution

Reconstitute in water

Analysis Note

The biological activiy is calculated by the dose-dependent proliferation of murine BALBC/3T3 cells. ED50 corresponds to a specific activity of at least 1 x 105 units/mg.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Sigma-Aldrich

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SRP3067

IGF-Binding Protein-3 human

Juan E Puche et al.
Journal of translational medicine, 10, 224-224 (2012-11-15)
Insulin-like growth factor I (IGF-I) is a polypeptide hormone produced mainly by the liver in response to the endocrine GH stimulus, but it is also secreted by multiple tissues for autocrine/paracrine purposes. IGF-I is partly responsible for systemic GH activities
Shu-Lin Liu et al.
Molecular cell, 71(6), 1092-1104 (2018-09-04)
Activation of class I phosphatidylinositol 3-kinase (PI3K) leads to formation of phosphatidylinositol-3,4,5-trisphophate (PIP3) and phosphatidylinositol-3,4-bisphophate (PI34P2), which spatiotemporally coordinate and regulate a myriad of cellular processes. By simultaneous quantitative imaging of PIP3 and PI34P2 in live cells, we here show
A comparison of the effects of equine luteinizing hormone (eLH), equine growth hormone (eGH) and human recombinant insulin-like growth factor (hrIGF-I) on steroid production in cultured equine Leydig cells during sexual maturation
Hess MF and Roser JF
Animal Reproduction Science, 89(1-4), 7-19 (2005)
An optimized system for studies of EPO-dependent murine pro-erythroblast development
Zhang D, et al.
Experimental Hematology, 29(11), 1278-1288 (2001)
M J E Walenkamp et al.
Endocrine development, 24, 128-137 (2013-02-09)
Molecular defects of the insulin-like growth factor 1 gene (IGF1) are rare in the human. Only three homozygous and two families with heterozygous mutations of the IGF1 gene have been described, resulting in a variable degree of intrauterine and postnatal
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