HT10132
Bouin′s solution
histological fixative
Synonym(s):
Bouin’s fluid
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About This Item
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Quality Level
shelf life
Expiry date on the label
composition
acetic acid, 5%
formaldehyde, 9%
picric acid, 0.9%
color
yellow
application(s)
hematology
histology
storage temp.
room temp
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General description
Bouin′s solution is a noncoagulant yellow stain fixative that consists of saturated picric acid, formaldehyde, and acetic acid.
Application
Bouin′s solution is an excellent fixative for preserving soft and delicate structures. It is used as a mordant in various trichrome procedures.
Additionally, it has been used for the following purposes-
Additionally, it has been used for the following purposes-
- staining of human liver biopsy tissue samples for two-photon excited fluorescence (TPEF) and second-harmonic generation (SHG) imaging
- fixation of rat ovaries for the observing follicle cells using microscopy
- fixation of gonads of crab for morphological and histological analysis
Prepared with saturated picric acid, formaldehyde and acetic acid. Excellent fixative for preserving soft and delicate structures. Used as a mordant in various trichrome procedures.
Biochem/physiol Actions
Bouin′s solution causes shrinkage of tissues when used as a preservative due to its picric acid component. It preserves morphological and nuclear information in tissues upon fixation.
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
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