H8629
Hexokinase and Glucose-6-phosphate Dehydrogenase from baker′s yeast (S. cerevisiae)
lyophilized powder
Synonym(s):
HK/G6PD
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About This Item
Recommended Products
form
lyophilized powder
Quality Level
glucose-6-phosphate dehydrogenase activity
≥75 units/mg protein (biuret)
hexokinase activity
≥65 units/mg protein
purified by
crystallization
storage temp.
−20°C
General description
Research area: Cell signaling
Hexokinase and Glucose-6-phosphate Dehydrogenase are intracellular enzymes found distributed widely in the living cells.
Hexokinase and Glucose-6-phosphate Dehydrogenase are intracellular enzymes found distributed widely in the living cells.
Enzyme Commission numbers 2.7.1.1 + 1.1.1.49
Application
Hexokinase and Glucose-6-phosphate Dehydrogenase from baker′s yeast (S. cerevisiae) has been used to evaluate the adenylate kinase (Adk), activities of the mutant Adk’s. It has also been used for the determination of glucose and ATP.
Biochem/physiol Actions
Hexokinase initiates the glycolysis pathway by catalyzing the phosphorylation of glucose to Glucose-6-phosphate in the presence of ATP. Glucose-6-phosphate dehydrogenase plays a role in the subsequent oxidation of Glucose-6-phosphate in the presence of triphosphopyridine nucleotide (TPN). Hexokinase and Glucose-6-phosphate Dehydrogenase both serve as a tool in biochemical and analytical studies for determining glucose, fructose, mannose, ATP, and creatin-kinase activity.
Packaging
Package sizes are based on hexokinase units.
Components
Mixture of hexokinase and glucose-6-phosphate dehydrogenase
Unit Definition
Hexokinase Unit Definition: One unit will phosphorylate 1.0 μmole of D-glucose per min at pH 7.6 at 25 °C.
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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